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Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

Submit a question References (9)
Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
  • Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

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APC

製品の概要

  • 製品名

    Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control
    Mouse アイソタイプ・コントロール 製品一覧

  • アプリケーション

    適用あり: Flow Cytmore details

  • 免疫原

    Synthetic peptide. Hapten from sequence of plant origin.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    Purified from supernatant from an in vitro culture.

  • ポリ/モノ

    モノクローナル

  • クローン名

    B12/8

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    kappa

  • 研究分野

  • 別名

    • Mouse Isotype Control

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab91361の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use 1µg for 106 cells.

ab91361 is a negative isotype control 
特記事項
Flow Cyt
Use 1µg for 106 cells.

ab91361 is a negative isotype control 

画像

  • Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
    Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

    Flow cytometry analysis of normal whole blood sample labeling IgG2a with ab91361 at 1μg. 

  • Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
    Flow Cytometry - Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

    Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

参考文献 (9)

ab91361 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab91361 は 9 報の論文で使用されています。

  • Giadans CG  et al. Intrahepatic immune infiltrate in chronic hepatitis B and chronic hepatitis C: Similar but not the same. J Viral Hepat 29:124-134 (2022). PubMed: 34820942
  • Mahajan AS  et al. Creatine and Nicotinamide Prevent Oxidant-Induced Senescence in Human Fibroblasts. Nutrients 13:N/A (2021). PubMed: 34836359
  • Benz PM  et al. AKAP12 deficiency impairs VEGF-induced endothelial cell migration and sprouting. Acta Physiol (Oxf) 228:e13325 (2020). PubMed: 31162891
  • Chen X  et al. Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8+ T cells activation via JAK-STAT pathway in vitiligo. Free Radic Biol Med 139:80-91 (2019). PubMed: 31078730
  • Rios DA  et al. Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis. Sci Rep 7:13283 (2017). PubMed: 29038590
  • Khan A  et al. Mesenchymal stem cells internalize Mycobacterium tuberculosis through scavenger receptors and restrict bacterial growth through autophagy. Sci Rep 7:15010 (2017). PubMed: 29118429
  • Abo Al Hayja M  et al. Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients. J Inflamm (Lond) 12:28 (2015). Flow Cyt ; Human . PubMed: 25969669
  • Jun E  et al. Design of a multicomponent peptide-woven nanocomplex for delivery of siRNA. PLoS One 10:e0118310 (2015). PubMed: 25705892
  • Tashima Y & Stanley P Antibodies that detect O-linked ß-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins. J Biol Chem 289:11132-42 (2014). Flow Cyt . PubMed: 24573683

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