AB280974

Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control

BOND RX™ Validated
Recombinant
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(4 Publications)

Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab280974) is a mouse monoclonal antibody used in Flow Cytometry (Intra), IHC-P, ICC/IF.

8 Images

Immunocytochemistry/ Immunofluorescence - Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB280974)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB280974)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling Mouse IgG1 with ab280974 at 1/20 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Confocal image showing no staining in RAW 264.7 cells.

Negative control 1 : ab280974 at a 1/20 dilution followed by ab150080 at a 1/1000 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

Flow Cytometry (Intracellular) - Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB280974)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB280974)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Mouse IgG1 with ab280974 at 1/500 dilution (0.1μg) (Red) compared with a Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control ab18443 (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB280974)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling Mouse IgG1 with ab280974 at 1/20 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Confocal image showing no staining in HeLa cells.

Negative control 1 : ab280974 at a 1/20 dilution followed by ab150113 at a 1/1000 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150080 at a 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB280974)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB280974)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Mouse IgG1 with ab280974 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab280974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

No staining on the mouse kidney.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB280974)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Mouse IgG1 with ab280974 at 1/100 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab280974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

No staining on the rat kidney.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB280974)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Mouse IgG1 with ab280974 at 1/100 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab280974 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

No staining on the human colon.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB280974)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Human cervix adenocarcinoma epithelial cell, Left) / PC-12 (Rat adrenal gland pheochromocytoma, Right) cells labelling Mouse IgG1 with ab280974 at 1/500 dilution (0.1μg) (Red) compared with a Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control ab18443 (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB280974)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling Mouse IgG1 with ab280974 at 1/20 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).

Confocal image showing no staining in PC-12 cells.

Negative control 1 : ab280974 at a 1/20 dilution followed by ab150080 at a 1/1000 dilution.

Negative control 2 : ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

R312-MouseIgG1

アイソタイプ

IgG1

キャリアフリー

アプリケーション

Flow Cyt (Intra), IHC-P, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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製品の詳細

What is this antibody validated in?
Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (ab280974) is a mouse recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF). Other related products
We have a range of other formats of antibody clone [R312-MouseIgG1] also available for your convenience: ab280974, Carrier free - ab281291, FITC - ab314952, PE - ab322670

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012725 PubMed39585899

2024

N6-methyladenosine RNA modification promotes Severe Fever with Thrombocytopenia Syndrome Virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Chen,Jinyu Zhang,Jun Wang,Hao Tong,Wen Pan,Feng Ma,Qihan Wu,Jianfeng Dai

JCI insight 8: PubMed37471168

2023

Pansclerotic morphea is characterized by IFN-γ responses priming dendritic cell fibroblast crosstalk to promote fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Enze Xing,Feiyang Ma,Rachael Wasikowski,Allison C Billi,Mehrnaz Gharaee-Kermani,Jennifer Fox,Craig Dobry,Amanda Victory,Mrinal K Sarkar,Xianying Xing,Olesya Plazyo,Henry W Chen,Grant Barber,Heidi Jacobe,Pei-Suen Tsou,Robert L Modlin,John Varga,J Michelle Kahlenberg,Lam C Tsoi,Johann E Gudjonsson,Dinesh Khanna

Aging and disease 14:484-501 PubMed37008056

2023

CO-Induced TTP Activation Alleviates Cellular Senescence and Age-Dependent Hepatic Steatosis Downregulation of .

Applications

Unspecified application

Species

Unspecified reactive species

Jeongmin Park,Yingqing Chen,Jeongha Kim,Eunyeong Hwang,Gyu Hwan Park,Chae Ha Yang,Stefan W Ryter,Jeong Woo Park,Hun Taeg Chung,Yeonsoo Joe

British journal of cancer 128:1466-1477 PubMed36759726

2023

Prevalence of tumour-infiltrating CD103 cells identifies therapeutic-sensitive prostate cancer with poor clinical outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Quan Zhou,Yuxi Ou,Xiyu Dai,Xinan Chen,Siqi Wu,Wensun Chen,Mengbo Hu,Chen Yang,Limin Zhang,Haowen Jiang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control