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Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (ab18443)

Q&A (2)References (55)

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製品の概要

  • 製品名

    Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control
    Mouse アイソタイプ・コントロール 製品一覧

  • 特異性

    MOPC-21 immunoglobulin has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live and fixed rat and human tissues.

  • アプリケーション

    適用あり: IHC-P, IHC-Fr, Flow Cyt, IP, WBmore details

  • 免疫原

    Other Immunogen Type corresponding to Mouse. There is no immunogen for this product because it was not generated through a hybridoma. It was generated by the myeloma of a mouse that was fed mineral oil.

  • 特記事項

    ab18443 MOPC-21 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • アイソタイプ・コントロール 備考

    ab18433 MOPC-21 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MOPC-21

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

  • 別名

    • Mouse Isotype Control

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18443の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

参考文献 (55)

ab18443 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18443 は 55 報の論文で使用されています。

  • Pant P & Kumarswamy R Multiple Oligo assisted RNA Pulldown via Hybridization followed by Mass Spectrometry (MORPH-MS) for exploring the RNA-Protein interactions. RNA Biol 21:1-9 (2024). PubMed: 38105541
  • Kovatcheva M  et al. Vitamin B12 is a limiting factor for induced cellular plasticity and tissue repair. Nat Metab 5:1911-1930 (2023). PubMed: 37973897
  • Ye D  et al. Co-enrichment of CD8-positive T cells and macrophages is associated with clinical benefit of tislelizumab in solid tumors. Biomark Res 11:25 (2023). PubMed: 36879284
  • Pintacuda G  et al. Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders. Cell Genom 3:100250 (2023). PubMed: 36950384
  • Pendse M  et al. Macrophages regulate gastrointestinal motility through complement component 1q. Elife 12:N/A (2023). PubMed: 37159507
View all Publications for this product

レビューと Q&A

Q&A
質問を送る

1-2 of 2 Q&A

Question

We purchased your IgG 1 Kappa control antibody (ab18443) about 4 months ago. I was wondering if you could tell me what the lot number would be for that timeframe.

We accidentally tossed the vial and I need it for a report I'm filling out. Thanks for your help!

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Answer

Thank you for contacting us.

If you provide me with the institution that placed the order, I will be able to tell you the exact lot number you purchased. However, roughly 4 months ago we were selling lot GR54839-4.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Need IgG isotype control for IHC on paraffin sections. I have 3 mouse monoclonal antibodies which I require isotype controls for - 2 mouse are type IgG1, one is IgG1,kappa. Do I need to match for the kappa as well or can I just use a IgG1? Please can you tell me whether I can just use IgG1 isotype control for all antibodies or does the IgG1, kappa require one of the other isotype controls, such as ab18443 or ab18447?

Read More
Answer

Thank you for your enquiry. According to my literature 99% of mouse IgG immunoglobulin molecules have a kappa light chain. This is reflected in the number of IgG, kappa isotype controls in our catalogue. Therefore given that your three mouse antibodies are IgG1; with the light chain unspecified in two of them and one specified as kappa I think that it is safe to assume that you can use a single isotype control relating for all three antibodies relating to heavy chain IgG1 and kappa light chain. For example; ab18443 Mouse IgG1, kappa monoclonal (MOPC-21) - isotype control or ab18447 Mouse IgG1, kappa monoclonal [MG1-45] - Isotype control. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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