Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
Related conjugates and formulations
製品の概要
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製品名
Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control
Mouse アイソタイプ・コントロール 製品一覧 -
アプリケーション
適用あり: Flow Cyt, IHC-P, IHC-Frmore details -
免疫原
Chemical/ Small Molecule corresponding to Mouse. Synthetic hapten, which is normally not present in humans or animals.
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特記事項
ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab91353 is purified and passed through a 0.22µm filter -
アイソタイプ・コントロール 備考
ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections -
ポリ/モノ
モノクローナル -
クローン名
B11/6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
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別名
- Mouse Isotype Control
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab91353の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 2µg for 106 cells.
ab91353 is a negative isotype control |
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use 2µg for 106 cells. ab91353 is a negative isotype control |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
画像
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Glomerular nestin expression in experimental groups.
(A) Negative control (×200).
(C) SHADR group: Diffuse glomerular nestin expression was detected involving almost all podocytes within glomerulus. After losartan and tempol treatment, either single or in combination, kidneys restored nestin expression similar to controls (SHC group).
Immunostaining was applied on 5 μm thick paraffin sections. After deparaffinization and rehydration, the sections were treated by microwave for 20 minutes at 400 W in citrate buffer (pH 6.0). After antigen retrieval, samples were incubated for 1 hour at room temperature with primary antibody for nestin (dilution 1:100). Sections were then treated using 3-amino-9-ethylcarbazole (AEC) as substrate, and counterstained with hematoxylin. Negative controls were performed by omitting the first antibody and mouse monoclonal antibodies as isotype control mouse IgG1 (ab91353) antibody was also used.
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Effects of hypoxia on mRNA expression of IGF1R and IGF1, and production of IGF1 in cancer cells.
Cell surface expression of IGF1R by FACScan analysis. IGF1R expression level of Panc-1, RWP-1, OCUP-AT, and MiaPaCa-2 cells was higher in hypoxia than that in normoxia.
Cells (2 x 106 cells/mL) were fixed with 2% paraformaldehyde and incubated in PBS with anti IGF1R antibody (ab16890, Abcam) or mouse IgG1- isotype control (ab91353, Abcam) for 30 minutes at 22°C. Cells were subsequently labeled with FITC-conjugated secondary antibody (1:500; ab96879, Abcam) for 30 minutes at 22°C.
The percentage of positive cells were calculated and compared with isotype-matched control-stained cells.
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Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab59509 (red line).
The cells were fixed with 80% methanol (5 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC.
Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (49)
ab91353 は 49 報の論文で使用されています。
- Fernández Vallone V et al. Spontaneous Osteoclastogenesis, a risk factor for bone metastasis in advanced luminal A-type breast cancer patients. Front Oncol 13:1073793 (2023). PubMed: 36890825
- Giadans CG et al. Intrahepatic immune infiltrate in chronic hepatitis B and chronic hepatitis C: Similar but not the same. J Viral Hepat 29:124-134 (2022). PubMed: 34820942
- Yuan B et al. Wiskott-Aldrich syndrome protein forms nuclear condensates and regulates alternative splicing. Nat Commun 13:3646 (2022). PubMed: 35752626
- Funakoshi A et al. Cholecystokinin Receptor Antagonist Suppresses Melanoma Growth by Inducing Apoptosis of Tumor Cells. JID Innov 2:100153 (2022). PubMed: 36262666
- Lederman ES et al. Histologic case series of human acellular dermal matrix in superior capsule reconstruction. J Shoulder Elbow Surg 30:2146-2155 (2021). PubMed: 33600900