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AB221848

Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free

Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free

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(4 Publications)

Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free Suitable for ICC/IF, Flow Cyt, ELISA and reacts with samples. Cited in 4 publications.
1 Images
Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free (AB221848)
  • Flow Cyt

Unknown

Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free (AB221848)

This flow cytometry data was generated using the same antibody clone under a different buffer format (cat# ab170190).

Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).

An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

関連する標識済み抗体及び組成の異なる製品 (3)

  • Unconjugated

    Anti-GAL4 antibody [15-6E10A7]

  • Unconjugated

    Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

15-6E10A7

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

Yes

アプリケーション

ELISA, IHC-P, ICC/IF, Flow Cyt

applications

特異性

<p>This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments.</p>

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代替製品

Primary Antibodies

AB221849

Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control - BSA and Azide free

  • 0
  • 0
Primary Antibodies

AB221850

Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free

Flow Cytometry - Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control - BSA and Azide free (AB221850)

  • 0
  • 0

組合せ製品

Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
  • 5
Sample Prep & Detection Kits

AB201801

DyLight® 594 Conjugation Kit (Fast) - Lightning-Link®

Fluorescence Microscopy - DyLight® 594 Conjugation Kit (Fast) - Lightning-Link® (AB201801)

  • 0
  • 0
Primary Antibodies

AB281291

Mouse IgG1 monoclonal [R312-MouseIgG1] - Isotype control - BSA and Azide free

BOND RX™ Validated|Recombinant

Immunocytochemistry/ Immunofluorescence - Mouse IgG1 monoclonal [R312-MouseIgG1] - Isotype control - BSA and Azide free (AB281291)

  • 0
  • 0
Primary Antibodies

AB18443

Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control

ChIP - Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control (AB18443)

  • 0
  • 0
Primary Antibodies

AB282458

Anti-PD-L1 antibody [RM1012]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [RM1012] (AB282458)

  • 0
  • 0
Primary Antibodies

AB170190

Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control

ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry paraffin embedded sections", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "IHCP-species-checked": "", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab221848 is a PBS only buffer formulated version of ab170190.

Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody's host species, isotype, and possible conjugation.

Immunogen
Recombinant full length protein. ab221848 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Precipitation Ammonium Sulphate
バッファー組成
pH: 7.5 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 6:23300 PubMed26996236

2016

The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation.

Applications

Flow Cyt

Species

Unspecified reactive species

Yi Cui,Jin Han,Zhifeng Xiao,Tong Chen,Bin Wang,Bing Chen,Sumei Liu,Sufang Han,Yongxiang Fang,Jianshu Wei,Xiujie Wang,Xu Ma,Jianwu Dai

The Journal of biological chemistry 291:1974-90 PubMed26627833

2015

Oxidative Stress Attenuates Lipid Synthesis and Increases Mitochondrial Fatty Acid Oxidation in Hepatoma Cells Infected with Hepatitis C Virus.

Applications

IHC-P

Species

Unspecified reactive species

Donna N Douglas,Christopher Hao Pu,Jamie T Lewis,Rakesh Bhat,Anwar Anwar-Mohamed,Michael Logan,Garry Lund,William R Addison,Richard Lehner,Norman M Kneteman

Cell and tissue banking 17:137-45 PubMed26220398

2015

Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Landa-Solís,Julio Granados-Montiel,Anell Olivos-Meza,Carmina Ortega-Sánchez,Mónica Cruz-Lemini,Cecilia Hernández-Flores,María Eugenia Chang-González,Ricardo Gómez García,Brenda Olivos-Díaz,María Cristina Velasquillo-Martínez,Carlos Pineda,Clemente Ibarra

Veterinary journal (London, England : 1997) 206:191-6 PubMed26364240

2015

Significance of caveolin-1 and matrix metalloproteinase 14 gene expression in canine mammary tumours.

Applications

IF

Species

Unspecified reactive species

M Ebisawa,H Iwano,M Nishikawa,Y Tochigi,T Komatsu,Y Endou,K Hirayama,H Taniyama,T Kadosawa,H Yokota
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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