Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
製品の概要
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製品名
Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control
Mouse アイソタイプ・コントロール 製品一覧 -
特異性
This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. -
アプリケーション
適用あり: Flow Cyt, ELISA, IHC-P, ICC/IFmore details -
免疫原
Recombinant full length protein. ab170190 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.
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特記事項
This antibody clone is manufactured by Abcam.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation. The control performed appropriately in all materials and platforms that were tested.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Ammonium Sulphate Precipitation -
ポリ/モノ
モノクローナル -
クローン名
15-6E10A7 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
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別名
- Mouse Isotype Control
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170190の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 1 µg/ml.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. PubMed: 26627833
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ICC/IF |
Use a concentration of 1 µg/ml.
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特記事項 |
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Flow Cyt
Use a concentration of 1 µg/ml. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 26627833 |
ICC/IF
Use a concentration of 1 µg/ml. |
画像
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Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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Immunocytochemistry (ICC) experiments with NIH-3T3 (mouse sarcoma), COS7 (monkey kidney fibroblast), H9C2 (rat myoblast) and Hela (human adenocarcinoma) were performed with the IgG1 Isotype Control Antibody (top), no primary antibody negative control (middle), and ab14705 as a positive control (bottom).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.
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Fluorescence ELISA.
An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (32)
ab170190 は 32 報の論文で使用されています。
- Jin Z & Liu Y The m6A reader YTHDC1-mediated lncRNA CTBP1-AS2 m6A modification accelerates cholangiocarcinoma progression. Heliyon 9:e19816 (2023). PubMed: 37809459
- Min Y et al. Cross species systems biology discovers glial DDR2, STOM, and KANK2 as therapeutic targets in progressive supranuclear palsy. Nat Commun 14:6801 (2023). PubMed: 37919278
- Peniche Silva CJ et al. Enthesis: not the same in each localisation - a molecular, histological and biomechanical study. Eur Cell Mater 44:43-55 (2022). PubMed: 35976149
- Yan B et al. SIRT3-Mediated CypD-K166 Deacetylation Alleviates Neuropathic Pain by Improving Mitochondrial Dysfunction and Inhibiting Oxidative Stress. Oxid Med Cell Longev 2022:4722647 (2022). PubMed: 36092157
- Jiang R et al. METTL3 stabilizes HDAC5 mRNA in an m6A-dependent manner to facilitate malignant proliferation of osteosarcoma cells. Cell Death Discov 8:179 (2022). PubMed: 35396379