Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control

• ELISA

Supplier Data

ELISA - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190)

Fluorescence ELISA.

An isotyping ELISA was performed by coating a 96-well plate with 1.25 ug/mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG. This experiment verifies that the primary antibody's isotype is correct and that it is successfully bound by the secondary antibody.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190)

Immunocytochemistry (ICC) experiments with NIH-3T3 (mouse sarcoma), COS7 (monkey kidney fibroblast), H9C2 (rat myoblast) and Hela (human adenocarcinoma) were performed with the IgG1 Isotype Control Antibody (top), no primary antibody negative control (middle), and ab14705 as a positive control (bottom).

An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.

• Flow Cyt

Unknown

Flow Cytometry - Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (AB170190)

Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).

An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.