Anti-MORC3 抗体 (ab76813)
Key features and details
- Mouse polyclonal to MORC3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MORC3 antibody
MORC3 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to MORC3 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human MORC3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- HeLa whole cell lysate (ab150035).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76813の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 107 kDa.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 107 kDa. |
ターゲット情報
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機能
Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism (PubMed:20501696). Sumoylated MORC3-NBs can also associate with PML-NBs (PubMed:20501696). Recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity (PubMed:17332504). Binds RNA in vitro (PubMed:11927593). May be required for influenza A transcription during viral infection (PubMed:26202233). -
組織特異性
Expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney. -
配列類似性
Contains 1 CW-type zinc finger. -
翻訳後修飾
Sumoylation is involved in interaction with PML and localization to PML nuclear bodies. -
細胞内局在
Nucleus, nucleoplasm. Nucleus matrix. Nucleus, PML body. Also found in PML-independent nuclear bodies. Localization to nuclear bodies is ATP-dependent. - Information by UniProt
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参照データベース
- Entrez Gene: 23515 Human
- Omim: 610078 Human
- SwissProt: Q14149 Human
- Unigene: 421150 Human
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別名
- KIAA0136 antibody
- Microrchidia 3 antibody
- MORC family CW type zinc finger 3 antibody
see all
画像
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Western blot - Anti-MORC3 antibody (ab76813)Anti-MORC3 antibody (ab76813) at 1/500 dilution + nuclear extracts from Hela cells at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size: 107 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted? -
Western blot - Anti-MORC3 antibody (ab76813)All lanes : Anti-MORC3 antibody (ab76813) at 1/500 dilution
Lane 1 : MORC3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size: 107 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (2)
ab76813 は 2 報の論文で使用されています。
- Ver LS et al. The Cellular Factor NXP2/MORC3 Is a Positive Regulator of Influenza Virus Multiplication. J Virol 89:10023-30 (2015). PubMed: 26202233
- Ichimura Y et al. Anti-NXP2 autoantibodies in adult patients with idiopathic inflammatory myopathies: possible association with malignancy. Ann Rheum Dis : (2012). WB ; Human . PubMed: 22258483
