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Anti-Monoamine Oxidase B/MAOB 抗体 (ab118865)

Reviews (2) Submit a question References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody (ab118865)
  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody (ab118865)

Key features and details

  • Goat polyclonal to Monoamine Oxidase B/MAOB
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Monoamine Oxidase B/MAOB antibody
    Monoamine Oxidase B/MAOB 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to Monoamine Oxidase B/MAOB

  • 由来種

    Goat

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Guinea pig, Cow, Dog, Pig

  • 免疫原

    Synthetic peptide corresponding to Human Monoamine Oxidase B/MAOB aa 300-400 (internal sequence).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Human brain cortex tissue, Human brain lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab118865の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 59 kDa.
IHC-P (1)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 59 kDa.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody (ab118865)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody (ab118865)

    ab118865 at 5 µg/ml staining Monoamine Oxidase B/MAOB in Paraffin-embedded Human brain cortex tissue by immunohistochemistry. After incubation with ab118865 slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody (ab118865)
    Western blot - Anti-Monoamine Oxidase B/MAOB antibody (ab118865)
    All lanes : Anti-Monoamine Oxidase B/MAOB antibody (ab118865) at 1 µg/ml

    Lane 1 : Human brain lysate without blocking peptide
    Lane 2 : Human brain lysate with blocking peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 59 kDa

参考文献 (2)

ab118865 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab118865 は 2 報の論文で使用されています。

  • Lim DW  et al. Chlorogenic Acid from Hawthorn Berry (Crataegus pinnatifida Fruit) Prevents Stress Hormone-Induced Depressive Behavior, through Monoamine Oxidase B-Reactive Oxygen Species Signaling in Hippocampal Astrocytes of Mice. Mol Nutr Food Res N/A:e1800029 (2018). PubMed: 29893510
  • Cha YJ  et al. Site-specific expression of amine oxidases in breast cancer metastases. Tumour Biol 40:1010428318776822 (2018). PubMed: 29775160

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Monoamine Oxidase B/MAOB antibody

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (BRAIN)
Permeabilization
Yes - 0.1 % TRITION X
Specification
BRAIN
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Pl Abhilash

Verified customer

投稿 Jun 15 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Monoamine Oxidase B/MAOB antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse brain(cortex))
Antigen retrieval step
None
Permeabilization
Yes - 0.2% Triton-x 100
Specification
mouse brain(cortex)
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 29 2012

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