AB259928

Anti-Monoamine Oxidase B/MAOB 抗体 [EPR24131-79]

Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal Monoamine Oxidase B/MAOB antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 5 publications.

別名を表示する

Amine oxidase [flavin-containing] B, Monoamine oxidase type B, MAO-B, Maob

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (AB259928)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (AB259928)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Monoamine Oxidase B with ab259928 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasm staining on mouse liver. The section was incubated with ab259928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (AB259928)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Monoamine Oxidase B with ab259928 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasm staining on rat liver. The section was incubated with ab259928 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (AB259928)

Monoamine Oxidase B was immunoprecipitated from 0.35 mg Mouse liver tissue lysate 10 ug with ab259928 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259928 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse liver tissue lysate 10 ug

Lane 2 : ab259928 IP in Mouse liver tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259928 in mouse liver tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds

Sample loaded onto lane 1 are non-boiled as boiling may cause protein aggregates.

All lanes:

Immunoprecipitation - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928)

Predicted band size: 59 kDa

Observed band size: 59 kDa

false

Lab

Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (AB259928)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID : 8058968).

Low expression : mouse testis.

Protein aggregates may be caused by boiling of samples .

Exposure time : 15 seconds

All lanes:

Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 59 kDa

Observed band size: 59 kDa

false

Lab

Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (AB259928)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (ab259928) at 1/1000 dilution

Lane 1:

E.coli extracts containing his-tagged mouse Amine oxidase flavin-containi B recombinant protein (aa 2-489) at 10 µL

Lane 2:

E.coli extracts containing his-tagged mouse Amine oxidase flavin-containi A recombinant protein (aa 1-497) at 4 µL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 59 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR24131-79

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat

アプリケーション

IP, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Monoamine oxidase B (MAOB) also known as MAO-B or MOA-B is a flavoenzyme that catalyzes the oxidative deamination of amines. It plays an important role in the catabolism of monoamines including neurotransmitters such as dopamine. The enzyme is approximately 58 kDa in size and is primarily expressed in the brain particularly in the basal ganglia region but also found in platelets and other tissues. MAOB exists in the outer mitochondrial membrane where it is closely associated with cellular respiration processes.

Biological function summary

Monoamine oxidase B aids in the regulation of neurotransmitter levels by breaking down dopamine phenylethylamine and other bioamines. It acts individually rather than forming part of larger protein complexes. This regulatory action influences several neurological processes contributing to the synaptic transmission and maintaining homeostasis in central nervous system functions. The activity of MAOB varies with age generally increasing over time which has implications for its role in aging-related neural changes.

Pathways

Monoamine oxidase B is involved in the dopamine metabolic pathway which impacts neurological functions and behaviors. It also connects to the phenylethylamine catabolic process influencing neuropsychiatric states. MAOB directly interacts with proteins like dopamine transporter proteins that influence dopamine reuptake and it works alongside monoamine oxidase A (MAOA) which degrades other neurotransmitters such as serotonin and norepinephrine providing a comprehensive regulatory mechanism within the monoamine system.

Monoamine oxidase B has a significant connection to neurodegenerative diseases such as Parkinson's disease and psychiatric disorders like depression. Its increased activity is linked to the neurodegeneration observed in Parkinson's due to excessive breakdown of dopamine. Through the process of oxidative stress MAOB contributes to neuronal damage particularly when interacting with proteins like α-synuclein aggravating the disease pathology. Additionally its regulation of dopamine levels can influence depressive states indicating potential therapeutic targets for MAOB inhibitors in these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the oxidative deamination of primary and some secondary amines such as neurotransmitters, and exogenous amines including the tertiary amine, neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), with concomitant reduction of oxygen to hydrogen peroxide and participates in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues (PubMed : 4156831). Preferentially degrades benzylamine and phenylethylamine (By similarity).

See full target information Maob

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cancers 17: PubMed40563592

2025

The Identification of a Key Regulator of Mitochondrial Metabolism, the LRPPRC Protein, as a Novel Therapeutic Target in SDHA-Overexpressing Ovarian Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Szulta,Lin Wang,Ameera Hasan,Michael Kinter,Atul Pranay,Jie Zhu,Kenneth M Humphries,Brooke Loveland,Timothy M Griffin,Magdalena Bieniasz

Molecular psychiatry 30:506-520 PubMed39122778

2024

Deletion of murine astrocytic vesicular nucleotide transporter increases anxiety and depressive-like behavior and attenuates motivation for reward.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Huang,Hiu Ham Lee,Bryan Volpe,Qingchen Zhang,Chang Xue,Brian C Liu,Yahia R Abuhasan,Lingyun Li,Jeremy S Yang,Julie Egholm,Cristina Gutierrez-Vazquez,Allen Li,Alyssa Lee,Sharon Tang,Chun Wa Wong,Tiemin Liu,Yuan Huang,Raddy L Ramos,Randy F Stout,Abdelfattah El Ouaamari,Francisco J Quintana,Bradford B Lowell,C Ronald Kahn,Emmanuel N Pothos,Weikang Cai

The Tohoku journal of experimental medicine 263:217-226 PubMed38839359

2024

Shaoyao Gancao Decoction Mitigates Helicobacter Pylori-Induced Chronic Atrophic Gastritis by Suppressing MAOB.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoyang Li,Xueming He,Chuming Liu

Neural regeneration research 19:2081-2088 PubMed38227539

2024

Gut microbiota dysbiosis contributes to α-synuclein-related pathology associated with C/EBPβ/AEP signaling activation in a mouse model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Fang,Sha Liu,Bilal Muhammad,Mingxuan Zheng,Xing Ge,Yan Xu,Shu Kan,Yang Zhang,Yinghua Yu,Kuiyang Zheng,Deqin Geng,Chun-Feng Liu

Evidence-based complementary and alternative medicine : eCAM 2022:9191284 PubMed36437828

2022

Therapeutic and Neuroprotective Effects of Bushen Jianpi Decoction on a Rotenone-Induced Rat Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Liang,Lulu Yao,Jiahao Chen,Zhiying Chen,Xiling Wu,Xiaobo Huang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Monoamine Oxidase B/MAOB 抗体 [EPR24131-79]

Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (AB259928)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (AB259928)

Immunoprecipitation - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (AB259928)

Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (AB259928)

Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR24131-79] (AB259928)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

組合せ製品

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise