Anti-Monoacylglycerol Lipase/MGL 抗体 (ab77398)
Key features and details
- Goat polyclonal to Monoacylglycerol Lipase/MGL
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Monoacylglycerol Lipase/MGL antibody
Monoacylglycerol Lipase/MGL 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Monoacylglycerol Lipase/MGL -
由来種
Goat -
特異性
abb77398 is expected to recognize both reported isoforms (NP_009214.1 and NP_001003794.1). Preliminary testing was unsuccessful on Rat.
-
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Dog
-
免疫原
Synthetic peptide corresponding to Human Monoacylglycerol Lipase/MGL aa 1-100 (internal sequence). (NP_001003794.1, NP_009214.1)
Database link: Q99685-1 -
ポジティブ･コントロール
- Flow Cyt (Intra): A431 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab77398 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77398の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
|
| IHC-P | (1) |
Use a concentration of 5 µg/ml.
|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).
Primary incubation 1 hour at room temperature. |
|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
IHC-P
Use a concentration of 5 µg/ml. |
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa). Primary incubation 1 hour at room temperature. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
-
機能
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. -
組織特異性
Detected in adipose tissue, lung, liver, kidney, brain and heart. -
パスウェイ
Glycerolipid metabolism; triacylglycerol degradation. -
配列類似性
Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family. - Information by UniProt
-
参照データベース
- Entrez Gene: 11343 Human
- Entrez Gene: 23945 Mouse
- Entrez Gene: 29254 Rat
- Omim: 609699 Human
- SwissProt: Q99685 Human
- SwissProt: O35678 Mouse
- SwissProt: Q8R431 Rat
- Unigene: 277035 Human
see all -
別名
- EC 3.1.1.23 antibody
- HU K5 antibody
- HU-K5 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398)
Immunocytochemistry/immunofluorescence analysis of paraformaldehyde fixed U2OS cells labelling Monoacylglycerol Lipase/MGL with ab77398. Cells were permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488® secondary antibody (2 µg/mL).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemical staining of Monoacylglycerol Lipase/MGL using ab77398 on Formaldehyde-fixed paraffin-embedded mouse adipose tissue sections (from around the kidney). Antigen retrieval step: heat mediated in citric acid HIER. Blocking step: 1% BSA for 10 mins @ rt°C. ab77398 incubated at 1/500 for 2 hours. Secondary Antibody: biotin labelled anti goat IgG used at 1/200. The submitted image shows white fat deposits around the kidney. Dilution factors vary between 1/300-500. There is a pale positivity in the renal tubular cytoplasm which appears to be specific (at lower dilution factor this is more obvious but, in this block the fat was too strongly immunostained). Section was endogenous biotin-blocked prior to incubation with primary Ab.
-
Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398)Anti-Monoacylglycerol Lipase/MGL antibody (ab77398) at 0.5 µg/ml + Mouse Adipose lysate (35 µg protein in RIPA buffer)
Predicted band size: 33 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
-
Flow Cytometry (Intracellular) - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
Immunocytochemistry/ Immunofluorescence - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398)Immunocytochemistry/immunofluorescence analysis of paraformaldehyde fixed A431 cells labelling Monoacylglycerol Lipase/MGL with ab77398. Cells were permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL), showing cytoplasmic and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488® secondary antibody (2 µg/mL).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoacylglycerol Lipase/MGL antibody (ab77398)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue labelling Monoacylglycerol Lipase/MGL with ab77398 at 5 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer pH 6. Counterstained with hematoxylin.
Inset: Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab77398 は 4 報の論文で使用されています。
- Liktor-Busa E et al. ABHD6 and MAGL control 2-AG levels in the PAG and allodynia in a CSD-induced periorbital model of headache. Front Pain Res (Lausanne) 4:1171188 (2023). PubMed: 37287623
- Li J et al. Purinergic 2X7 receptor is involved in adipogenesis and lipid degradation. Exp Ther Med 23:81 (2022). PubMed: 34934450
- Zhang A et al. Lactate-induced M2 polarization of tumor-associated macrophages promotes the invasion of pituitary adenoma by secreting CCL17. Theranostics 11:3839-3852 (2021). PubMed: 33664865
- Nielsen JE et al. Characterisation and localisation of the endocannabinoid system components in the adult human testis. Sci Rep 9:12866 (2019). PubMed: 31537814