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AB248009

Anti-Monoacylglycerol Lipase/MGL 抗体 [EPR6339] - BSA and Azide free

Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Monoacylglycerol Lipase/MGL antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

Monoglyceride lipase, MGL, HU-K5, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase, MAGL, MGLL

2 Images
Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] - BSA and Azide free (AB248009)
  • WB

Unknown

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] - BSA and Azide free (AB248009)

This data was developed using ab124796, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] (<a href='/products/primary-antibodies/monoacylglycerol-lipase-mgl-antibody-epr6339-ab124796'>ab124796</a>) at 1/1000 dilution

All lanes:

Human heart lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

false

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] - BSA and Azide free (AB248009)
  • WB

Unknown

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] - BSA and Azide free (AB248009)

This data was developed using ab124796, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339] (<a href='/products/primary-antibodies/monoacylglycerol-lipase-mgl-antibody-epr6339-ab124796'>ab124796</a>) at 1/1000 dilution

All lanes:

Human adipose tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Unconjugated

    Anti-Monoacylglycerol Lipase/MGL antibody [EPR6339]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6339

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

ab248009 is the carrier-free version of ab124796.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Monoacylglycerol lipase (MGL) also known as MAGL or lipase 63 is an enzyme with a mass of approximately 33 kDa. MGL primarily functions in lipid metabolism by hydrolyzing 2-monoacylglycerol (2-MAG) into free fatty acids and glycerol. This enzyme is expressed in many tissues with high expression in the brain liver and adipose tissue. Its activity plays a significant role in the breakdown of monoacylglycerol an important intermediate in the endocannabinoid signaling system.
Biological function summary

MGL contributes to the regulation of energy homeostasis by controlling the levels of 2-monoacylglycerol. It catalyzes the final step in the degradation of the endocannabinoid 2-arachidonoylglycerol (2-AG) which is important for modulating inflammation pain and mood. MGL is not known to be part of a larger protein complex. In the brain its action helps terminate endocannabinoid signaling therefore influencing neurotransmission and synaptic plasticity.

Pathways

MGL plays a critical role in the endocannabinoid and glycerolipid metabolism pathways. In the endocannabinoid system it interacts with the cannabinoid receptors CB1 and CB2 by regulating 2-AG levels. The protein's role in glycerolipid metabolism involves its interaction with long-chain acyl-CoA synthetase and fatty acid oxidation pathways which are essential for maintaining lipid balance and cellular energy.

MGL has been linked to neurodegenerative diseases and cancer. In neurodegenerative diseases like Alzheimer’s dysregulation of MGL activity may lead to altered endocannabinoid signaling impacting neuronal health. In cancer increased MGL activity can promote tumor progression by providing cancer cells with fatty acids for growth and survival. MGL's interaction with proteins such as fatty acid synthase highlights its importance in cancer metabolism.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Converts monoacylglycerides to free fatty acids and glycerol (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (PubMed : 20079333).
See full target information MGLL
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0190722 PubMed36946735

2023

Characterization of the Impact of Merkel Cell Polyomavirus-Induced Interferon Signaling on Viral Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ranran Wang,June F Yang,Taylor E Senay,Wei Liu,Jianxin You
View all publications
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組合せ製品

Assay Kits

AB273326

Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)

Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (AB273326)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

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