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AB415

Anti-mono methyl Arginine 抗体 [5D1]

Anti-mono methyl Arginine antibody [5D1]

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(7 Publications)

Mouse Monoclonal mono methyl Arginine antibody. Suitable for ChIP, WB and reacts with Modified Amino Acid samples. Cited in 7 publications.

別名を表示する

Arginine (mono methyl)

2 Images
ChIP - Anti-mono methyl Arginine antibody [5D1] (AB415)
  • ChIP

Unknown

ChIP - Anti-mono methyl Arginine antibody [5D1] (AB415)

Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab415 to mono methyl Arginine.

Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5 μl of ab415 and 4x106 cells were used in each ChIP experiment.

This image is courtesy of Sylvain Daujat, Tony Kouzarides lab, Cambridge University, UK

ChIP - Anti-mono methyl Arginine antibody [5D1] (AB415)
  • ChIP

Unknown

ChIP - Anti-mono methyl Arginine antibody [5D1] (AB415)

We looked at chicken chromatin and then analysed 4 active genes (AG1-4), 2 inactive genes (IG1-2) as well as heterochromatin (Het). A "Yes" indicates that the antibody immunoprecipitated material well above background and should be regarded as a positive result.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

5D1

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

No

アプリケーション

WB, ChIP

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "5 µL for 10^6 Cells", "ChIP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Mono methyl arginine also known as MMA is a post-translational modification where a single methyl group is added to the guanidino nitrogen group of arginine residues. This modification is catalyzed by a group of enzymes called protein arginine methyltransferases (PRMTs). MMA exists in proteins throughout various tissues mainly in the nucleus cytoplasm and sometimes in mitochondria. This modification contributes to the molecular mass of modified proteins which varies depending on the protein itself.
Biological function summary

The process of arginine methylation which includes mono methyl arginine formation plays a role in diverse cellular functions such as signal transduction RNA processing and gene expression regulation. Mono methyl arginine often participates in forming larger protein complexes. For instance it can act as a binding site for other proteins in these complexes facilitating interactions necessary for cellular operations. It is important in the regulation of transcription factors and also influences DNA repair mechanisms.

Pathways

Mono methyl arginine is integral in the regulation of the epigenetic pathways and cellular signaling pathways such as the Ras signaling pathway. By altering the methylation status of proteins it affects protein-protein interactions and the activity of signaling molecules. This modification is closely associated with proteins like histones in epigenetic regulation and the protein Ras in signaling pathways. Through these associations it influences important cellular decisions such as differentiation proliferation and apoptosis.

Mono methyl arginine modifications have linkages with cancer and cardiovascular disorders. In cancer abnormal arginine methylation can lead to dysregulation of gene expression promoting oncogenesis. Proteins such as PRMT1 are often found deregulated in cancerous cells. In cardiovascular diseases where altered vascular tone is a significant factor mono methyl arginine has associations with endothelial dysfunction. This dysfunction can further lead to changes in the regulation of the protein endothelial nitric oxide synthase (eNOS) affecting cardiovascular health.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information mono methyl Arginine
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36293180

2022

PRMT7 Inhibitor SGC8158 Enhances Doxorubicin-Induced DNA Damage and Its Cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Ahyeon Jeong,Yena Cho,Minkyeong Cho,Gyu-Un Bae,Dae-Geun Song,Su-Nam Kim,Yong Kee Kim

Cell 184:3915-3935.e21 PubMed34174187

2021

The single-cell epigenomic and transcriptional landscape of immunity to influenza vaccination.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Wimmers,Michele Donato,Alex Kuo,Tal Ashuach,Shakti Gupta,Chunfeng Li,Mai Dvorak,Mariko Hinton Foecke,Sarah E Chang,Thomas Hagan,Sanne E De Jong,Holden T Maecker,Robbert van der Most,Peggie Cheung,Mario Cortese,Steven E Bosinger,Mark Davis,Nadine Rouphael,Shankar Subramaniam,Nir Yosef,Paul J Utz,Purvesh Khatri,Bali Pulendran

eLife 9: PubMed32844749

2020

Arginine methylation of SHANK2 by PRMT7 promotes human breast cancer metastasis through activating endosomal FAK signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Yingqi Liu,Lingling Li,Xiaoqing Liu,Yibo Wang,Lingxia Liu,Lu Peng,Jiayuan Liu,Lian Zhang,Guannan Wang,Hongyuan Li,Dong-Xu Liu,Baiqu Huang,Jun Lu,Yu Zhang

Cell 173:1385-1397.e14 PubMed29706550

2018

Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Peggie Cheung,Francesco Vallania,Hayley C Warsinske,Michele Donato,Steven Schaffert,Sarah E Chang,Mai Dvorak,Cornelia L Dekker,Mark M Davis,Paul J Utz,Purvesh Khatri,Alex J Kuo

Biopolymers 101:133-43 PubMed23576281

2013

Chemical and biological methods to detect post-translational modifications of arginine.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J Slade,Venkataraman Subramanian,Jakob Fuhrmann,Paul R Thompson

Journal of molecular biology 367:1118-29 PubMed17303166

2007

Methylation of arginine residues interferes with citrullination by peptidylarginine deiminases in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Reinout Raijmakers,Albert J W Zendman,Wilma Vree Egberts,Erik R Vossenaar,Jos Raats,Claudia Soede-Huijbregts,Floris P J T Rutjes,Peter A van Veelen,Jan W Drijfhout,Ger J M Pruijn

Cell 118:545-53 PubMed15339660

2004

Histone deimination antagonizes arginine methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Graeme L Cuthbert,Sylvain Daujat,Andrew W Snowden,Hediye Erdjument-Bromage,Teruki Hagiwara,Michiyuki Yamada,Robert Schneider,Philip D Gregory,Paul Tempst,Andrew J Bannister,Tony Kouzarides
View all publications
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Alternative Products

Primary Antibodies

AB414

Anti-mono methyl Arginine antibody [16B11]

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Complementary Products

Primary Antibodies

AB412

Anti-mono and dimethyl Arginine antibody [7E6]

ChIP - Anti-mono and dimethyl Arginine antibody [7E6] (AB412)

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Primary Antibodies

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Anti-Histone H2A.Z antibody - ChIP Grade

Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (AB4174)

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Primary Antibodies

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Anti-Histone H4 antibody - ChIP Grade

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Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

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