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AB129466

Anti-mnt 抗体

Anti-mnt antibody

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(1 Publication)

Rabbit Polyclonal MNT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MNT aa 1-50.

別名を表示する

BHLHD3, ROX, MNT, Max-binding protein MNT, Class D basic helix-loop-helix protein 3, Myc antagonist MNT, Protein ROX, bHLHd3

2 Images
Immunoprecipitation - Anti-mnt antibody (AB129466)
  • IP

Unknown

Immunoprecipitation - Anti-mnt antibody (AB129466)

Detection of mnt by Western Blot of Immunoprecipitates.

ab129466 at 1μg/ml staining mnt in HeLa whole cell lysate immunoprecipitated using ab129466 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-mnt antibody (ab129466)

Predicted band size: 62 kDa

false

Western blot - Anti-mnt antibody (AB129466)
  • WB

Unknown

Western blot - Anti-mnt antibody (AB129466)

All lanes:

Western blot - Anti-mnt antibody (ab129466) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 62 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IP, WB

applications

免疫原

Synthetic Peptide within Human MNT aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q99583

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
ab129466 is affinity purified using an epitope specific to mnt immobilized on solid support.
バッファー組成
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 'mnt' protein known as Max's Next Tango is a transcriptional repressor with a significant role in gene expression regulation. Mnt has a molecular mass of approximately 34 kDa and belongs to the basic helix-loop-helix (bHLH) family. It forms homodimers or heterodimers with Max another member of the bHLH family. Mnt is expressed in several tissues and cell types including liver brain and muscle tissue. Its presence in diverse tissues indicates a multifaceted role in basic cellular function.
Biological function summary

Mnt functions to regulate cell proliferation and differentiation by acting as a negative regulator. Mnt often forms a complex with Max to repress the transcription of Myc target genes. Myc proteins promote cell cycle progression and growth while Mnt exerts an opposing effect by working as a transcriptional repressor. This delicate balance between Myc activation and Mnt repression is necessary to maintain controlled cell proliferation.

Pathways

Mnt regulates essential cellular processes through the Myc/Max/Mad network and the E2F transcription factor pathway. Within the Myc/Max/Mad network mnt balances out the activity of Myc by forming a competing dimer with Max. In its interaction with the E2F pathway mnt indirectly influences cell cycle progression. These pathways include numerous proteins like Mad1 and Mad3 which also interact with Mnt to modulate transcription.

Mnt has a recognized connection with cancer and neurodegenerative diseases. Abnormal expression or mutations in mnt can disrupt the balance of cell proliferation often leading to tumorigenesis. In neurodegenerative diseases improper regulation of Myc and Mnt affects neurogenesis and neuronal survival. Proteins like p53 often implicated in these diseases show interconnected pathways with Mnt highlighting its potential as a therapeutic target.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'.
See full target information MNT
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of women's health 17:1165-1174 PubMed40308269

2025

MiR-484 Regulates the IL-6/STAT3 Signaling Pathway by Down-Regulating KLF12 to Inhibit the Malignant Progression of Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Keyong Bao,Xue Zhang,Lihong Bao,Xiaoyu Tao,Li Zhang,Dong Wang
View all publications
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組合せ製品

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