Anti-MNAT1 抗体 [EPR2555(2)] (ab154802)
![Western blot - Anti-MNAT1 antibody [EPR2555(2)] (ab154802)](https://www.abcam.co.jp/ps/products/154/ab154802/Images/MAT1A-Primary-antibodies-ab154802-1.jpg "Western blot - Anti-MNAT1 antibody [EPR2555(2)] (ab154802)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2555(2)] to MNAT1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MNAT1 antibody [EPR2555(2)]
MNAT1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2555(2)] to MNAT1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide within Human MNAT1. The exact sequence is proprietary.
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ポジティブ･コントロール
- A431, HeLa, A673 and Raw264.7 cell lysates; HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR2555(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154802の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
|
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| ICC/IF |
1/100 - 1/250.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
|
ICC/IF
1/100 - 1/250. |
追加情報
Is unsuitable for IHC-P or IP.
ターゲット情報
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機能
Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. -
組織特異性
Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes. -
配列類似性
Contains 1 RING-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4331 Human
- Entrez Gene: 17420 Mouse
- Entrez Gene: 266713 Rat
- Omim: 602659 Human
- SwissProt: P51948 Human
- SwissProt: P51949 Mouse
- Unigene: 509523 Human
- Unigene: 246750 Mouse
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別名
- MNAT 1 antibody
- CAP35 antibody
- CDK activating kinase assembly factor MAT1 antibody
see all
画像
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Western blot - Anti-MNAT1 antibody [EPR2555(2)] (ab154802)All lanes : Anti-MNAT1 antibody [EPR2555(2)] (ab154802) at 1/1000 dilution
Lane 1 : A431 lysate
Lane 2 : HeLa lysate
Lane 3 : A673 lysate
Lane 4 : RAW264.7 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody [EPR2555(2)] (ab154802)](https://www.abcam.co.jp/ps/products/154/ab154802/Images/MAT1A-Primary-antibodies-ab154802-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MNAT1 antibody [EPR2555(2)] (ab154802)Immunofluorescence analysis of HeLa cells labeling MAT1 with ab154802 at 1/100 dilution. -
![Flow Cytometry (Intracellular) - Anti-MNAT1 antibody [EPR2555(2)] (ab154802)](https://www.abcam.co.jp/ps/products/154/ab154802/Images/MAT1A-Primary-antibodies-ab154802-5.jpg)
Flow Cytometry (Intracellular) - Anti-MNAT1 antibody [EPR2555(2)] (ab154802)Intracellular flow cytometric analysis of permeabilized Hela cells labeling MAT1 with ab154802 at 1/10 dilution (red) or a negative rabbit IgG (green).
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![Anti-MNAT1 antibody [EPR2555(2)] (ab154802)](https://www.abcam.co.jp/ps/products/154/ab154802/Images/ab154802-4-benefits-of-recombinant-antibodies.png)
Anti-MNAT1 antibody [EPR2555(2)] (ab154802)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154802 は論文での使用が確認できていません。