Anti-MMS22L (C6ORF167) 抗体 [EPR13654] (ab181047)
![Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.co.jp/ps/products/181/ab181047/Images/ab181047-478712-anti-mms22l-c6orf167-antibody-epr13654-westernblot-human-mouse-rat.jpg "Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13654] to MMS22L (C6ORF167)
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MMS22L (C6ORF167) antibody [EPR13654]
MMS22L (C6ORF167) 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13654] to MMS22L (C6ORF167) -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa, K562 and HCT-116 whole cell lysate; K562 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13654 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181047の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 142 kDa).
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| IP |
1/20.
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| 特記事項 |
|---|
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 142 kDa). |
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IP
1/20. |
ターゲット情報
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機能
Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. -
配列類似性
Belongs to the MMS22 family. MMS22L subfamily. -
細胞内局在
Nucleus. Localizes to DNA damage sites, accumulates at stressed replication forks. - Information by UniProt
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参照データベース
- Entrez Gene: 253714 Human
- Entrez Gene: 212377 Mouse
- Entrez Gene: 313108 Rat
- Omim: 615614 Human
- SwissProt: Q6ZRQ5 Human
- SwissProt: B1AUR6 Mouse
- Unigene: 444292 Human
- Unigene: 120919 Mouse
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別名
- C6orf167 antibody
- C6orf167 chromosome 6 open reading frame 167 antibody
- chromosome 6 open reading frame 167 antibody
see all
画像
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Western blot - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)All lanes : Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 3 : LLC1 (Mouse lung carcinoma ) whole cell lysate
Lane 4 : Mouse testis lysate
Lane 5 : Mouse spleen lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 142 kDa -
![Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.co.jp/ps/products/181/ab181047/Images/ab181047-7-anti-mms22l-c6orf167-antibody-epr13654-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)Purified ab181047 at 1:20 dilution (0.5µg) immunoprecipitating MMS22L (C6ORF167) in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg.
Lane 2 (+): ab181047 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181047 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1:5000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: kDa -
![Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.co.jp/ps/products/181/ab181047/Images/ab181047-6-anti-mms22l-c6orf167-antibody-epr13654-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling MMS22L (C6ORF167) with Purified ab181047 at 1:20 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)](https://www.abcam.co.jp/ps/products/181/ab181047/Images/ab181047-4-benefits-of-recombinant-antibodies.png)
Anti-MMS22L (C6ORF167) antibody [EPR13654] (ab181047)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (4)
ab181047 は 4 報の論文で使用されています。
- Guo Z et al. Integrative pan-cancer landscape of MMS22L and its potential role in hepatocellular carcinoma. Front Genet 13:1025970 (2022). PubMed: 36276962
- Luo Q et al. MMS22L Expression as a Predictive Biomarker for the Efficacy of Neoadjuvant Chemoradiotherapy in Oesophageal Squamous Cell Carcinoma. Front Oncol 11:711642 (2021). PubMed: 34660277
- Sun H et al. Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2. PLoS Genet 15:e1007685 (2019). PubMed: 30779731
- Huang TH et al. The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells. Mol Cell 69:879-892.e5 (2018). PubMed: 29478807