Anti-MMS21 抗体 (ab169355)
Key features and details
- Mouse polyclonal to MMS21
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MMS21 antibody
MMS21 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to MMS21 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow
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免疫原
Recombinant full length protein within Human MMS21 aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- Recombinant Human MMS21 protein (ab132298) can be used as a positive control in WB. MMS21 transfected 293T cell lysate; HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab169355の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair. -
パスウェイ
Protein modification; protein sumoylation. -
配列類似性
Belongs to the NSE2 family.
Contains 1 SP-RING-type zinc finger. -
翻訳後修飾
Sumoylated, possibly via autosumoylation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 519974 Cow
- Entrez Gene: 286053 Human
- SwissProt: Q32KY9 Cow
- SwissProt: Q96MF7 Human
- Unigene: 388297 Human
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別名
- C8orf36 antibody
- E3 SUMO-protein ligase NSE2 antibody
- hMMS21 antibody
see all
画像
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Western blot - Anti-MMS21 antibody (ab169355)All lanes : Anti-MMS21 antibody (ab169355) at 1 µg/ml
Lane 1 : MMS21 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 28 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MMS21 antibody (ab169355)Immunofluorescent analysis of HeLa cells labeling MMS21with ab169355 at 10 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab169355 は論文での使用が確認できていません。
