Anti-MMP9 抗体 (ab38898)
Key features and details
- Rabbit polyclonal to MMP9
- Suitable for: WB
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MMP9 antibody
MMP9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MMP9 -
由来種
Rabbit -
特異性
The antibody binds to MMP-9, but does not cross react with the other MMP family members (MMP-1, MMP-2, MMP-3).
In our hands, no signal or a very weak signal was observed in WB in native human samples (BLAST of full length mouse protein sequence showed 72% homology with the Human MMP9 sequence).
Treatment increasing the expression of MMP-9 is recommended when using this antibody.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Recombinant fragment -
免疫原
Full length protein corresponding to Mouse MMP9.
Database link: P41245 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38898の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (20) |
1/1000. Detects a band of approximately 92 kDa. When using colorimetric substrates such as BCIP/NBT use at a 1/5000 dilution (for chemiluminescent substrates). Detects a band of approximately 92-95 kDa (pro-form) and 82kDa (active form) (Human samples). Mouse MMP9 is larger, and on SDS PAGE gels runs about 102-105 kDa. Dilution optimised using Chromogenic detection.
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| 特記事項 |
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WB
1/1000. Detects a band of approximately 92 kDa. When using colorimetric substrates such as BCIP/NBT use at a 1/5000 dilution (for chemiluminescent substrates). Detects a band of approximately 92-95 kDa (pro-form) and 82kDa (active form) (Human samples). Mouse MMP9 is larger, and on SDS PAGE gels runs about 102-105 kDa. Dilution optimised using Chromogenic detection. |
ターゲット情報
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機能
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
組織特異性
Produced by normal alveolar macrophages and granulocytes. -
関連疾患
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
配列類似性
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
細胞内局在
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 17395 Mouse
- SwissProt: P41245 Mouse
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別名
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
see all
画像
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Western blot - Anti-MMP9 antibody (ab38898)Anti-MMP9 antibody (ab38898) + Human MMP9
Observed band size: 88,92 kDa why is the actual band size different from the predicted?
Ab38898 detects a band at 92 Kd (pro-form) and a band at 88 Kd (active form). Mouse MMP9 is slightly larger than human MMP9, and the antibody detects a band at about 105 Kd. It is recommend to concentrate samples by Gelatin-agarose affinity chromatography prior to Western blot usage. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1034)
ab38898 は 1034 報の論文で使用されています。
- Zhang G et al. 1-2 T static magnetic field combined with Ferumoxytol prevent unloading-induced bone loss by regulating iron metabolism in osteoclastogenesis. J Orthop Translat 38:126-140 (2023). PubMed: 36381248
- Farini A et al. Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin-deficient mice. EMBO Mol Med 15:e16244 (2023). PubMed: 36533294
- Li X & Wu F Mesenchymal stem cell-derived extracellular vesicles transfer miR-598 to inhibit the growth and metastasis of non-small-cell lung cancer by targeting THBS2. Cell Death Discov 9:3 (2023). PubMed: 36609437
- Karreman MA et al. Active Remodeling of Capillary Endothelium via Cancer Cell-Derived MMP9 Promotes Metastatic Brain Colonization. Cancer Res 83:1299-1314 (2023). PubMed: 36652557
- Kurose S et al. Interleukin-38 suppresses abdominal aortic aneurysm formation in mice by regulating macrophages in an IL1RL2-p38 pathway-dependent manner. Physiol Rep 11:e15581 (2023). PubMed: 36708509