Anti-MMP9 抗体 [56-2A4] (ab58803)
![Western blot - Anti-MMP9 antibody [56-2A4] (ab58803)](https://www.abcam.co.jp/ps/products/58/ab58803/Images/ab58803-218393-ab58803.jpg "Western blot - Anti-MMP9 antibody [56-2A4] (ab58803)")
Key features and details
- Mouse monoclonal [56-2A4] to MMP9
- Suitable for: IHC-P, IHC-Fr, ICC/IF, WB
- Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-MMP9 antibody [56-2A4]
MMP9 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [56-2A4] to MMP9 -
由来種
Mouse -
特異性
Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP9. Does not react with MMP1, MMP2, MMP3 or MMP13. The antibody recognizes precursor (92 kDa) and active (83 kDa) forms of human and guinea pig MMP-9. -
アプリケーション
適用あり: IHC-P, IHC-Fr, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Guinea pig, Human -
免疫原
Synthetic peptide corresponding to Human MMP9 aa 626-644.
Sequence:LFSGRRLWRFDVKAQMVDP
Database link: P14780 -
ポジティブ･コントロール
- Breast carcinoma tissue. Conditioned medium of human fibrosarcoma HT1080 cells The protein is secreted so you may need to concentrate the protein from cell culture media. Concentrate protein by TCA, acetone or ammonium sulphate precipitation. HL60 cell lysate, U937 cell lysate, HT1080 cell lysate, human spleen lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.1% Sodium azide
Constituents: 1.64% Sodium phosphate, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Is purified. -
ポリ/モノ
モノクローナル -
クローン名
56-2A4 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58803の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (3) |
Use at an assay dependent concentration.
|
| IHC-Fr | (3) |
Use at an assay dependent concentration.
|
| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 22496779
|
| WB | (2) |
Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa).
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| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 22496779 |
|
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa). |
ターゲット情報
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機能
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
組織特異性
Produced by normal alveolar macrophages and granulocytes. -
関連疾患
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
配列類似性
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
細胞内局在
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 4318 Human
- Entrez Gene: 17395 Mouse
- Entrez Gene: 81687 Rat
- Omim: 120361 Human
- SwissProt: P14780 Human
- SwissProt: P41245 Mouse
- SwissProt: P41246 Rabbit
- SwissProt: P50282 Rat
see all -
別名
- 82 kDa matrix metalloproteinase-9 antibody
- 92 kDa gelatinase antibody
- 92 kDa type IV collagenase antibody
see all
画像
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Western blot - Anti-MMP9 antibody [56-2A4] (ab58803)
1:500 dilution of ab58803 detected MMP-9 on 10 µg of Human spleen lysates. Arrow indicates protein MMP-9 (~92 kDa). Lower bands indicate active forms of MMP-9
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (114)
ab58803 は 114 報の論文で使用されています。
- Mishra N et al. Establishing a Dexamethasone Treatment Regimen To Alleviate Sulfur Mustard-Induced Corneal Injuries in a Rabbit Model. J Pharmacol Exp Ther 388:469-483 (2024). PubMed: 37316330
- Qiu C et al. A novel rabbit model of abdominal aortic aneurysm: Construction and evaluation. Heliyon 9:e17279 (2023). PubMed: 37389075
- Song X et al. Knockdown of CD44 inhibits proliferation, migration, and invasiveness in hepatocellular carcinoma cells by modulating CXCR4/Wnt/β-Catenin Axis. Acta Biochim Pol 70:117-122 (2023). PubMed: 36735564
- Li J et al. Hydrogen attenuates postoperative pain through Trx1/ASK1/MMP9 signaling pathway. J Neuroinflammation 20:22 (2023). PubMed: 36737785
- Oh HH et al. A disintegrin and metalloprotease 12 contributes to colorectal cancer metastasis by regulating epithelial‑mesenchymal transition. Int J Oncol 62:N/A (2023). PubMed: 36866761