Anti-MMP8 抗体 (ab53017)
Key features and details
- Rabbit polyclonal to MMP8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MMP8 antibody
MMP8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MMP8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide derived from human MMP8
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53017の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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| IHC-P | (2) |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
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機能
Can degrade fibrillar type I, II, and III collagens. -
組織特異性
Neutrophils. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
細胞内局在
Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules. - Information by UniProt
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参照データベース
- Entrez Gene: 4317 Human
- Entrez Gene: 17394 Mouse
- Omim: 120355 Human
- SwissProt: P22894 Human
- SwissProt: O70138 Mouse
- Unigene: 161839 Human
- Unigene: 16415 Mouse
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別名
- CLG 1 antibody
- CLG1 antibody
- Collagenase 1 antibody
see all
画像
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Western blot - Anti-MMP8 antibody (ab53017)All lanes : Anti-MMP8 antibody (ab53017) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells with no immunizing peptide
Lane 2 : Extracts from NIH/3T3 cells with immunizing peptide
Predicted band size: 53 kDa
Observed band size: 53 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP8 antibody (ab53017)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP8 antibody at 1/50 dilution. Left hand image; untreated tissue, Right hand image; tissue treated with the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-MMP8 antibody (ab53017)ICC/IF image of ab63017 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53017, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab53017 は 15 報の論文で使用されています。
- Zhang Y et al. Neutrophil infiltration leads to fetal growth restriction by impairing the placental vasculature in DENV-infected pregnant mice. EBioMedicine 95:104739 (2023). PubMed: 37544202
- Zhang C et al. Targeted Inhibition of Matrix Metalloproteinase-8 Prevents Aortic Dissection in a Murine Model. Cells 11:N/A (2022). PubMed: 36291087
- Winsnes A et al. Similar collagen distribution in full-thickness skin grafts in intraperitoneal and onlay positions, an experimental mice-study. Hernia 26:1695-1705 (2022). PubMed: 36048398
- Silva RCL et al. Diacerein-induced interleukin-1β deficiency reduces the inflammatory infiltrate and immunoexpression of matrix metalloproteinase-8 in periodontitis in rat molars. J Periodontol 93:1540-1552 (2022). PubMed: 35184279
- Duncan HF et al. The Critical Role of MMP13 in Regulating Tooth Development and Reactionary Dentinogenesis Repair Through the Wnt Signaling Pathway. Front Cell Dev Biol 10:883266 (2022). PubMed: 35531096