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リコンビナントRabMAb

Anti-MMP7 抗体 [EPR26105-26] (BSA and Azide free) (ab302894)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)
  • Immunohistochemistry (Frozen sections) - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)
  • Dot Blot - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)
  • Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26105-26] to MMP7 - BSA and Azide free
  • Suitable for: Dot blot, IHC-P, IHC-Fr
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free)
    MMP7 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26105-26] to MMP7 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Dot blot, IHC-P, IHC-Frmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IP or WB

  • 種交差性

    交差種: Mouse
    非交差種: Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse: small intestine, colon tissue. IHC-Fr: Mouse small intestine (fresh). DB: Mouse MMP7 peptide

  • 特記事項

    ab302894 is a carrier free version of ab302893. 

    ab302893 does not react in: WB with human, mouse, and rat samples; IHC-P with human and rat tissues; ICC/IF, intracellular flow cytometry, and IP with mouse tissues. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302894の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
特記事項
Dot blot
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IP or WB.

ターゲット情報

  • 機能

    Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.

  • 配列類似性

    Belongs to the peptidase M10A family.

  • ドメイン

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Matrilysin antibody
      • Matrin antibody
      • Matrix Metalloproteinase 7 antibody
      • Matrix metalloproteinase-7 antibody
      • MMP 7 antibody
      • MMP-7 antibody
      • MMP7 antibody
      • MMP7_HUMAN antibody
      • MPSL1 antibody
      • PUMP 1 antibody
      • Pump 1 protease antibody
      • Pump-1 protease antibody
      • PUMP1 antibody
      • Uterine matrilysin antibody
      • Uterine metalloproteinase antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)

      This data was developed using ab302893, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling MMP7 with ab302893 at 1/500 dilution (1.068 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on Paneth cells of mouse small intestine is observed. The section was incubated with ab302893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)

      This data was developed using ab302893, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling MMP7 with ab302893 at 1/500 dilution (1.068 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Negative control: no staining on mouse colon. The section was incubated with ab302893 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Immunohistochemistry (Frozen sections) - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)
      Immunohistochemistry (Frozen sections) - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)

      This data was developed using ab302893, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine (fresh) tissue labeling MMP7 with ab302893 at 1/100 dilution (5.34 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml) (Green). Positive staining in the Paneth cells of mouse small intestine was observed. The nuclear counterstain was DAPI (Blue). The section was incubated with ab302893 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). 

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution (2 µg/ml). 

    • Dot Blot - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)
      Dot Blot - Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)

      This data was developed using ab302893, the same antibody clone in a different buffer formulation. 

      Dot blot analysis of MMP7 using ab302893 at 1:1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

      Lane 1: Mouse MMP7 peptide

      Exposure time: 180 seconds

      Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

    • Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)
      Anti-MMP7 antibody [EPR26105-26] (BSA and Azide free) (AB302894)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302894 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302894 は論文での使用が確認できていません。

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