Anti-MMP23 抗体 [EPR7769] (ab181118)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP23 antibody [EPR7769] (ab181118)](https://www.abcam.co.jp/ps/products/181/ab181118/Images/ab181118-213433-ab181118IHC.JPG "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP23 antibody [EPR7769] (ab181118)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7769] to MMP23
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MMP23 antibody [EPR7769]
MMP23 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7769] to MMP23 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal brain tissue lysate, Calu 3 cell lysate, SK OV 3 cell lysate, Human testis tissue, A549 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7769 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181118の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 44 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
1/50 - 1/100.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 44 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Protease. May regulate the surface expression of some potassum channels by retaining them in the endoplasmic reticulum. -
組織特異性
Predominantly expressed in ovary, testis and prostate. -
配列類似性
Belongs to the peptidase M10A family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
ドメイン
The toxin-like domain associates with, and blocks several potassium channels in the nanomolar to low micromolar range. The relative affinity is Kv1.6 > Kv1.3 > Kv1.1 = Kv3.2 > Kv1.4. -
翻訳後修飾
N-glycosylated.
Proteolytic cleavage might yield an active form. -
細胞内局在
Endoplasmic reticulum membrane. Membrane. A secreted form produced by proteolytic cleavage may also exist. - Information by UniProt
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参照データベース
- Entrez Gene: 8510 Human
- Entrez Gene: 8511 Human
- Entrez Gene: 26561 Mouse
- Entrez Gene: 94339 Rat
- Omim: 603320 Human
- Omim: 603321 Human
- SwissProt: O75900 Human
- SwissProt: O88676 Mouse
see all -
別名
- Femalysin antibody
- Matrix metallopeptidase 21 antibody
- Matrix metallopeptidase 23A (pseudogene) antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP23 antibody [EPR7769] (ab181118)
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling MMP23 with ab181118 at 1/250.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-MMP23 antibody [EPR7769] (ab181118)](https://www.abcam.co.jp/ps/products/181/ab181118/Images/ab181118-213434-ab181118IF.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-MMP23 antibody [EPR7769] (ab181118)Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling MMP23 with ab181118 at 1/100 followed by fluorescence labelled Goat anti-rabbit IgG at 1/200 and counterstained with Dapi.
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![Western blot - Anti-MMP23 antibody [EPR7769] (ab181118)](https://www.abcam.co.jp/ps/products/181/ab181118/Images/ab181118-213432-ab181118WB.JPG)
Western blot - Anti-MMP23 antibody [EPR7769] (ab181118)All lanes : Anti-MMP23 antibody [EPR7769] (ab181118) at 1/1000 dilution
Lane 1 : Calu 3 cell lysate
Lane 2 : SK OV 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-MMP23 antibody [EPR7769] (ab181118)](https://www.abcam.co.jp/ps/products/181/ab181118/Images/ab181118-213431-ab181118WB1.JPG)
Western blot - Anti-MMP23 antibody [EPR7769] (ab181118)Anti-MMP23 antibody [EPR7769] (ab181118) at 1/1000 dilution + Human fetal brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 44 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted? -
![Anti-MMP23 antibody [EPR7769] (ab181118)](https://www.abcam.co.jp/ps/products/181/ab181118/Images/ab181118-5-benefits-of-recombinant-antibodies.png)
Anti-MMP23 antibody [EPR7769] (ab181118)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab181118 は論文での使用が確認できていません。