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リコンビナントRabMAb

Anti-MMP20 抗体 [EP1275Y] (ab76109)

Submit a review Q&A (1)References (4)
Western blot - Anti-MMP20 antibody [EP1275Y] (ab76109)
  • Anti-MMP20 antibody [EP1275Y] (ab76109)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1275Y] to MMP20
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MMP20 antibody [EP1275Y]
    MMP20 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1275Y] to MMP20

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human MMP20 aa 450-550 (C terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • WB: HeLa cell lysates, Mouse and Rat heart lysates.

  • 特記事項

    The production method for this product has been changed from hybridoma to recombinant on 15th April 2024

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76109の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
特記事項
WB
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation.

  • 組織特異性

    Expressed specifically in the enamel organ.

  • 関連疾患

    Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2) [MIM:612529]. AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.

  • 配列類似性

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.

  • ドメイン

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • 翻訳後修飾

    Autoactivates at least at the 107-Asn-
    -Tyr-108 site.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AI2A2 antibody
      • Enamel metalloproteinase antibody
      • Enamelysin antibody
      • Matrix metalloproteinase 20 antibody
      • Matrix metalloproteinase-20 antibody
      • MMP 20 antibody
      • MMP-20 antibody
      • MMP20 antibody
      • MMP20_HUMAN antibody
      see all

    画像

    • Western blot - Anti-MMP20 antibody [EP1275Y] (ab76109)
      Western blot - Anti-MMP20 antibody [EP1275Y] (ab76109)
      All lanes : Anti-MMP20 antibody [EP1275Y] (ab76109) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Rat heart lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 54 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: Lane 1 and Lane 2: 20 seconds, Lane 3: 3 seconds

    • Anti-MMP20 antibody [EP1275Y] (ab76109)
      Anti-MMP20 antibody [EP1275Y] (ab76109)

    参考文献 (4)

    ab76109 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab76109 は 4 報の論文で使用されています。

    • Liang Y  et al. Effect of canonical NF-?B signaling pathway on the differentiation of rat dental epithelial stem cells. Stem Cell Res Ther 10:139 (2019). PubMed: 31109359
    • Li Z  et al. Bcl11b regulates enamel matrix protein expression and dental epithelial cell differentiation during rat tooth development. Mol Med Rep 15:297-304 (2017). PubMed: 27959403
    • Yang T  et al. High amounts of fluoride induce apoptosis/cell death in matured ameloblast-like LS8 cells by downregulating Bcl-2. Arch Oral Biol 58:1165-73 (2013). WB ; Mouse . PubMed: 23598055
    • Mohazab L  et al. Critical role for avß6 integrin in enamel biomineralization. J Cell Sci 126:732-44 (2013). PubMed: 23264742

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    previous lot OK
    stored at -20ºC, as recommended
    WB : no band
    ICC/IF no staining
    dilutions as recommended

    Read More
    Answer

    Merci de nous avoir contactés.

    Nous sommes désolés d'apprendre que le produit ab76109que vous avez reçu ne fonctionne pas comme attendu.
    Comme convenu ce matin par téléphone, j'ai mis en place l'envoi d'une unité de ab76109 d'un lot différent de celui que vous avez originalement reçu. Le numéro de commande de remplacement gratuit de ce produit est : *******. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

    N'hésitez pas àme contacter lors d'une prochaine occasion.

    Bonne continuation dans vos recherches.

    Read More

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