Anti-MMP13 抗体 (ab84594)
Key features and details
- Rabbit polyclonal to MMP13
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MMP13 antibody
MMP13 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MMP13 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse
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免疫原
Synthetic peptide corresponding to Human MMP13 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93499) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84594の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 54 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 54 kDa). |
ターゲット情報
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機能
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. -
組織特異性
Seems to be specific to breast carcinomas. -
関連疾患
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 4322 Human
- Entrez Gene: 17386 Mouse
- Entrez Gene: 171052 Rat
- Omim: 600108 Human
- SwissProt: O18927 Horse
- SwissProt: P45452 Human
- SwissProt: P33435 Mouse
- SwissProt: O62806 Rabbit
see all -
別名
- CLG 3 antibody
- CLG3 antibody
- Collagenase 3 antibody
see all
画像
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Western blot - Anti-MMP13 antibody (ab84594)All lanes : Anti-MMP13 antibody (ab84594) at 1 µg/ml
Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
The bands observed at 46 kDa could potentially be a cleaved form of Human Collagenase 3 precursor. The first 19 amino acids in the sequence act as a signal peptide and the following 84 (A.A 20-103) act as a activation peptide. Once cleaved this gives the target protein an expected weight of 43 kDa.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab84594 は 26 報の論文で使用されています。
- Li G et al. Teriparatide ameliorates articular cartilage degradation and aberrant subchondral bone remodeling in DMM mice. J Orthop Translat 38:241-255 (2023). PubMed: 36514714
- Supolikova M et al. Murine herpesvirus-68-related growth factors treatment correlates with decrease of p53 and HIF-1α protein levels. Pathog Dis 81:N/A (2023). PubMed: 36997335
- Zhu Y et al. MiR-760 targets HBEGF to control cartilage extracellular matrix degradation in osteoarthritis. J Orthop Surg Res 18:186 (2023). PubMed: 36894989
- Jiang H et al. Innate/Inflammatory Bioregulation of Surfactant Protein D Alleviates Rat Osteoarthritis by Inhibiting Toll-Like Receptor 4 Signaling. Front Immunol 13:913901 (2022). PubMed: 35865531
- Yan J et al. Selonsertib Alleviates the Progression of Rat Osteoarthritis: An in vitro and in vivo Study. Front Pharmacol 12:687033 (2021). PubMed: 34322020