Anti-MMP10 抗体 (ab38930)
Key features and details
- Rabbit polyclonal to MMP10
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MMP10 antibody
MMP10 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MMP10 -
由来種
Rabbit -
特異性
The antibody binds to the hinge region of MMP10. The antibody binds to Stromelysin 2, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, etc.). The antibody also recognizes the cleaved active enzyme and will also bind to the non-reduced protein. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide based on the hinge region of the human MMP10 sequence.
Immunogen の所有権に関して (Peptide available as ab41075.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38930の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
| 特記事項 |
|---|
追加情報
IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Although the sequence homology for this portion of MMP10 is well conserved, higher concentrations of antibody may be needed for non-human samples. When used against the reduced protein the antibody identifies bands at 56 kDa (the pro-form), as well as the initial active forms (46 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Although the sequence homology for this portion of MMP10 is well conserved, higher concentrations of antibody may be needed for non-human samples. When used against the reduced protein the antibody identifies bands at 56 kDa (the pro-form), as well as the initial active forms (46 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 4319 Human
- Omim: 185260 Human
- SwissProt: P09238 Human
- Unigene: 2258 Human
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別名
- Matrix metallopeptidase 10 (stromelysin 2) antibody
- Matrix metalloprotease 10 antibody
- Matrix metalloproteinase 10 (stromelysin 2) antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP10 antibody (ab38930)Ab38930 staining Human normal trachea. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab38930 は 8 報の論文で使用されています。
- Baraibar-Churio A et al. Deficiency of MMP-10 Aggravates the Diseased Phenotype of Aged Dystrophic Mice. Life (Basel) 11:N/A (2021). PubMed: 34947929
- Wang Y et al. Long non-coding RNA OIP5-AS1 suppresses multiple myeloma progression by sponging miR-27a-3p to activate TSC1 expression. Cancer Cell Int 20:155 (2020). PubMed: 32410883
- Furuya H et al. Prognostic Significance of Lymphocyte Infiltration and a Stromal Immunostaining of a Bladder Cancer Associated Diagnostic Panel in Urothelial Carcinoma. Diagnostics (Basel) 10:N/A (2019). PubMed: 31905599
- Brilha S et al. Early Secretory Antigenic Target-6 Drives Matrix Metalloproteinase-10 Gene Expression and Secretion in Tuberculosis. Am J Respir Cell Mol Biol 56:223-232 (2017). WB ; Human . PubMed: 27654284
- Ehlken C et al. Increased expression of angiogenic and inflammatory proteins in the vitreous of patients with ischemic central retinal vein occlusion. PLoS One 10:e0126859 (2015). IHC . PubMed: 25978399
- Zhang G et al. Validation and clinicopathologic associations of a urine-based bladder cancer biomarker signature. Diagn Pathol 9:200 (2014). IHC ; Human . PubMed: 25387487
- Garcia-Irigoyen O et al. Matrix metalloproteinase-10 expression is induced during hepatic injury and plays a fundamental role in liver tissue repair. Liver Int 34:e257-70 (2014). PubMed: 24119197
- Frederick LA et al. Matrix metalloproteinase-10 is a critical effector of protein kinase Ciota-Par6alpha-mediated lung cancer. Oncogene 27:4841-53 (2008). IHC-P . PubMed: 18427549