AB137332

Anti-MMP1 抗体

Name: Anti-MMP1 抗体 (ab137332) | Abcam
Brand: Abcam Limited
SKU: AB137332
Price: 65000 JPY
Availability: InStock
Rating: 4 (12 reviews)

Anti-MMP1 antibody

(12 Reviews)

(95 Publications)

Anti-MMP1 antibody (ab137332) is a rabbit polyclonal antibody detecting MMP1 in Western Blot, ICC/IF. Suitable for Human, Mouse.

- Over 70 publications

別名を表示する

CLG, MMP1, Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1

3 Images

Supplier Data

Western blot - Anti-MMP1 antibody (AB137332)

Low expression : A549, HT-29, IMR-32.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 25319362).

Anti-MMP1 antibody (ab318207, Left) staining at 1/1000 dilution showed similar expression profile/ molecular weight with Anti-MMP1 antibody (ab137332, Right) at 1/1000 dilution.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MMP1 antibody [RM1206] (<a href='/products/primary-antibodies/mmp1-antibody-rm1206-ab318207'>ab318207</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa treated with 80 nM TPA for 24 hours, then replaced with 10 ug /ml poly(dA:dT) for 2 hours, whole cell lysate at 20 µg

Lane 3:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 54 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Immunoprecipitation - Anti-MMP1 antibody (AB137332)

MMP1 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab318207 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318207 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2 : ab318207 IP in HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318207 in HUVEC whole cell lysate

All lanes:

Immunoprecipitation - Anti-MMP1 antibody [RM1206] (<a href='/products/primary-antibodies/mmp1-antibody-rm1206-ab318207'>ab318207</a>) at 1/30 dilution

All lanes:

HUVEC (human umbilical vein endothelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 6s

Supplier Data

Western blot - Anti-MMP1 antibody (AB137332)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-MMP1 antibody (ab137332) at 1/2500 dilution

All lanes:

HUVEC Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 50 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human, Mouse

アプリケーション

WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human MMP1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P03956

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-MMP1 antibody (ab137332) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of MMP1?
Anti-MMP1 (ab137332) specifically detects a band for MMP1 (UniProt: P03956) at a molecular weight of 54kDa.

Trusted by the scientific community
Anti-MMP1 (ab137332) was first used in a scientific publication in 2012 and has been cited over 70 times in peer-reviewed journals.

Reviewed by scientists
Anti-MMP1 (ab137332) has over 10 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MMP1 also known as collagenase-1 is an enzyme belonging to the matrix metalloproteinase (MMP) family. It is responsible for the degradation of type I II and III collagens making it an important player in collagen turnover. MMP1 has a molecular weight of approximately 54 kDa. This enzyme is expressed in various tissues including skin lung and reproductive organs primarily during tissue remodeling and repair. Scientists often use MMP1 ELISA kits and MMP1 inhibitors to study its expression and activity levels.

Biological function summary

MMP1 plays a significant role in the modulation of the extracellular matrix. It facilitates cellular processes by breaking down structural proteins and allowing for cell migration proliferation and differentiation. Although it acts independently MMP1 works in harmony with other MMP family members to maintain extracellular matrix homeostasis and is often linked to tissue remodeling complexes. This makes the MMP1 a valuable target for researchers interested in tissue repair and fibrosis.

Pathways

Studies show MMP1 involvement in critical processes such as the inflammatory response and wound healing. In the inflammatory pathway MMP1 expression is regulated by cytokines that respond to tissue injury. Within the wound healing pathway this enzyme interacts with proteins like transforming growth factor-beta (TGF-beta) to coordinate the repair process by remodeling extracellular matrix components. This illustrates the interconnected nature of MMP1 with significant regulatory proteins.

MMP1 has significant associations with osteoarthritis and cancer metastasis. MMP1 contributes to the breakdown of cartilage in osteoarthritis highlighting its role in disease progression and joint degradation. In the context of cancer elevated MMP1 expression correlates with the metastatic potential where it assists tumor invasion by degrading the surrounding matrix. MMP1's involvement with other MMPs including MMP2 and MMP9 highlights its importance in these pathological conditions making it a therapeutic target in related research.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed : 1645757, PubMed : 2153297, PubMed : 2557822). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed : 16807369).

See full target information MMP1

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文献 (95)

Recent publications for all applications. Explore the full list and refine your search

Plastic and reconstructive surgery. Global open 13:e7060 PubMed40821896

2025

Novel Concept of "Right-at-the-right-place" Transplantation: Rejuvenating Effects of Mouse Fascial Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Ryoga Wake,Kento Takaya,Rikako Matsuura,Shigeki Sakai,Keisuke Okabe,Noriko Aramaki-Hattori,Kazuo Kishi

Annals of dermatology 37:152-161 PubMed40432364

2025

Electrical Stimulation Using a Low-Frequency and Low-Intensity Alternating Current Modulates Type I Procollagen Production and MMP-1 Expression in Dermal Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Mi Kang,Jung Min Ahn,Jieun Kim,Kyungho Paik,Bo Ri Kim,Dong Hun Lee,Sang Woong Youn,Keun-Yong Eom,Chong Won Choi

Journal of biomedical science 31:51 PubMed38741091

2024

Somatic PDGFRB activating variants promote smooth muscle cell phenotype modulation in intracranial fusiform aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Li Hao,Xiaolong Ya,Jiaye Wu,Chuming Tao,Ruochen Ma,Zhiyao Zheng,Siqi Mou,Yiming Ling,Yingxi Yang,Jiguang Wang,Yan Zhang,Qing Lin,Jizong Zhao

Aging 16:7915-7927 PubMed38728237

2024

IL21 inhibits miR-361-5p to promote MAP3K9 and further aggravate the progression of shoulder arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Kangning Hao,Pengchao Lin,Jing Li,Jie Hu,Jiangyong Wang,Fei Li

Biomedicines 12: PubMed38540123

2024

Antiadhesive Hyaluronic Acid-Based Wound Dressings Promote Wound Healing by Preventing Re-Injury: An In Vivo Investigation.

Applications

Unspecified application

Species

Unspecified reactive species

Da Som Kim,Keum-Yong Seong,Hyeseon Lee,Min Jae Kim,Sung-Min An,Jea Sic Jeong,So Young Kim,Hyeon-Gu Kang,Sangsoo Jang,Dae-Youn Hwang,Sung-Baek Seo,Seong-Min Jo,Seung Yun Yang,Beum-Soo An

Cell death discovery 10:65 PubMed38320998

2024

IL-8 activates fibroblasts to promote the invasion of HNSCC cells via STAT3-MMP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Chen,Li Huang,Rui-Huan Gan,Shuo Yuan,Ting Lan,Dali Zheng,You-Guang Lu

Biomaterials science 12:1477-1489 PubMed38294258

2024

Nitric oxide-scavenging hyaluronic acid nanoparticles for osteoarthritis treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Yunyoung Nah,Sivasangu Sobha,Gurusamy Saravanakumar,Byung-Yoon Kang,Joo-Byoung Yoon,Won Jong Kim

Aging 15:15525-15534 PubMed38159254

2024

M-CSF secreted by gastric cancer cells exacerbates the progression of gastric cancer by increasing the expression of SHP2 in tumor-associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohua Zhang,Dongfei Ren,Huiyu Hou,Li Yao,Hufang Yuan

Journal of nanobiotechnology 21:350 PubMed37759249

2023

Programmable DNA hydrogel provides suitable microenvironment for enhancing autophagy-based therapies in intervertebral disc degeneration treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Song Qingxin,Jiang Kai,Zheng Dandan,Jin Linyu,Chen Xiuyuan,Feng Yubo,Wang Kun,Han Yingchao,Chen Hao,Song Jie,Chen Zhi,Shen Hongxing

Photochemistry and photobiology 100:622-632 PubMed37732548

2023

Alleviation of ultraviolet-B radiation-induced photoaging using Saussurea medusa Maxim polysaccharide.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Guo,Yue Kong,Juan Sun,Yang Jiao,Yan Hong,Yan Wang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MMP1 抗体

Anti-MMP1 antibody

Western blot - Anti-MMP1 antibody (AB137332)

Immunoprecipitation - Anti-MMP1 antibody (AB137332)

Western blot - Anti-MMP1 antibody (AB137332)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (95)

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Abcam product promise