Anti-MLD 抗体 [EPR9680] (ab185237)
![Western blot - Anti-MLD antibody [EPR9680] (ab185237)](https://www.abcam.co.jp/ps/products/185/ab185237/Images/ab185237-412769-anti-mld-antibody-epr9680-western-blot-human-knockout-lysate.jpg "Western blot - Anti-MLD antibody [EPR9680] (ab185237)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9680] to MLD
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MLD antibody [EPR9680]
MLD 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9680] to MLD -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HepG2, U87-MG, 293 and T47D cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9680 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185237の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa).
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa). |
ターゲット情報
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関連性
DEGS1 is a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. It contains three His containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It has sphingolipid-delta-4-desaturase activity and converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine). -
細胞内局在
Endoplasmic reticulum and Mitochondrial -
参照データベース
- Entrez Gene: 8560 Human
- Omim: 615843 Human
- SwissProt: O15121 Human
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別名
- Degenerative spermatocyte homolog 1 antibody
- Degenerative spermatocyte homolog 1 lipid desaturase antibody
- Degenerative spermatocyte homolog lipid desaturase antibody
see all
画像
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Western blot - Anti-MLD antibody [EPR9680] (ab185237)All lanes : Anti-MLD antibody [EPR9680] (ab185237) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : DEGS1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDaLanes 1-4: Merged signal (red and green). Green - ab185237 observed at 38 kDa. Red - loading control ab8245 observed at 36 kDa.
ab185237 Anti-MLD antibody [EPR9680] was shown to specifically react with MLD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266481 (knockout cell lysate ab257918) was used. Wild-type and MLD knockout samples were subjected to SDS-PAGE. ab185237 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-MLD antibody [EPR9680] (ab185237)](https://www.abcam.co.jp/ps/products/185/ab185237/Images/ab185237-218658-anti-degs1-antibody-epr9680-western-blot.jpg)
Western blot - Anti-MLD antibody [EPR9680] (ab185237)All lanes : Anti-MLD antibody [EPR9680] (ab185237) at 1/10000 dilution
Lane 1 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa -
![Western blot - Anti-MLD antibody [EPR9680] (ab185237)](https://www.abcam.co.jp/ps/products/185/ab185237/Images/ab185237-218612-anti-degs1-antibody-epr9680-western-blot.jpg)
Western blot - Anti-MLD antibody [EPR9680] (ab185237)All lanes : Anti-MLD antibody [EPR9680] (ab185237) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : T47D cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa -
![Anti-MLD antibody [EPR9680] (ab185237)](https://www.abcam.co.jp/ps/products/185/ab185237/Images/ab185237-4-benefits-of-recombinant-antibodies.png)
Anti-MLD antibody [EPR9680] (ab185237)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (4)
ab185237 は 4 報の論文で使用されています。
- Thompson D et al. Fenretinide inhibits obesity and fatty liver disease but induces Smpd3 to increase serum ceramides and worsen atherosclerosis in LDLR-/- mice. Sci Rep 13:3937 (2023). PubMed: 36894641
- Alsanafi M et al. Dihydroceramide Desaturase Functions as an Inducer and Rectifier of Apoptosis: Effect of Retinol Derivatives, Antioxidants and Phenolic Compounds. Cell Biochem Biophys 79:461-475 (2021). PubMed: 33991313
- Alsanafi M et al. The regulation of p53, p38 MAPK, JNK and XBP-1s by sphingosine kinases in human embryonic kidney cells. Biochim Biophys Acta Mol Cell Biol Lipids 1865:158631 (2020). PubMed: 31954175
- Alsanafi M et al. Native and Polyubiquitinated Forms of Dihydroceramide Desaturase Are Differentially Linked to Human Embryonic Kidney Cell Survival. Mol Cell Biol 38:N/A (2018). PubMed: 30224516