Anti-MKLP1 抗体 [EPR10879] (ab174304)

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Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] (ab174304)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10879] to MKLP1
Suitable for: ICC/IF, WB
Reacts with: Mouse, Rat, Human

Alexa Fluor® 647 Carrier Free

製品名

Anti-MKLP1 antibody [EPR10879]
MKLP1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR10879] to MKLP1
由来種

Rabbit
アプリケーション

適用あり: ICC/IF, WBmore details
適用なし: IHC-P
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide within Human MKLP1 aa 800-900 (Cysteine residue). The exact sequence is proprietary.
Database link: Q02241
ポジティブ･コントロール
- HeLa, 293T and A549 cell lysates. A547 cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR10879
アイソタイプ

IgG
研究分野

Alternative Versions
- Alexa Fluor® 647 Anti-MKLP1 antibody [EPR10879] (ab204478)
- Anti-MKLP1 antibody [EPR10879] - BSA and Azide free (ab240178)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab174304の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ICC/IF		1/200 - 1/400.
WB		1/1000. Predicted molecular weight: 110 kDa.

特記事項
ICC/IF 1/200 - 1/400.
WB 1/1000. Predicted molecular weight: 110 kDa.

追加情報

Is unsuitable for IHC-P.

機能

Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro. Localizes to the interzone of mitotic spindles.
配列類似性

Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain.
細胞内局在

Nucleus. Cytoplasm > cytoskeleton > spindle.
Target information above from: UniProt accession Q02241 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 9493 Human
- Entrez Gene: 71819 Mouse
- Entrez Gene: 315740 Rat
- Omim: 605064 Human
- SwissProt: Q02241 Human
- Unigene: 270845 Human
別名
- CHO 1 antibody
- CHO1 antibody
- KIF 23 antibody
- KIF23 antibody
- KIF23_HUMAN antibody
- Kinesin family member 23 antibody
- Kinesin like 5 antibody
- Kinesin like protein 5 antibody
- Kinesin like protein KIF 23 antibody
- Kinesin like protein KIF23 antibody
- Kinesin-like protein 5 antibody
- Kinesin-like protein KIF23 antibody
- KNS L5 antibody
- KNSL 5 antibody
- KNSL5 antibody
- Mitotic kinesin like 1 antibody
- Mitotic kinesin like protein 1 antibody
- Mitotic kinesin-like protein 1 antibody
- MKLP 1 antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] (ab174304)

Immunofluorescence staining of HeLa cells with purified ab174304 at a working dilution of 1 in 400, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor^® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor^® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab174304 was used at a dilution of 1/200 followed by an Alexa Fluor^® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.
Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)

Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/5000 dilution (purified) + RAW264.7 cell lysate at 20 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 110 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)

Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/1000 dilution (purified) + Rat lung tissue lysate at 20 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 110 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)

Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/20000 dilution (purified) + HeLa cell lysate at 10 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 110 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)

All lanes : Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/5000 dilution (purified)

Lane 1 : HEK293 cell lysate
Lane 2 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 110 kDa
Observed band size: 110 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-MKLP1 antibody [EPR10879] (ab174304)

All lanes : Anti-MKLP1 antibody [EPR10879] (ab174304) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A549 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 110 kDa
Additional bands at: 98-110 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-MKLP1 antibody [EPR10879] (ab174304)

Immunofluorescent analysis of A547 cells labeling MKLP1 with unpurified ab174304 at 1/100.
Anti-MKLP1 antibody [EPR10879] (ab174304)

Western blot protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

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Datasheet download

Download

ab174304 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab174304 は 9 報の論文で使用されています。

Wu Z et al. Inhibition of KIF23 Alleviates IPAH by Targeting Pyroptosis and Proliferation of PASMCs. Int J Mol Sci 23:N/A (2022). PubMed: 35457254
Xu J et al. Knockdown of circ_0067934 inhibits gastric cancer cell proliferation, migration and invasion via the miR‑1301‑3p/KIF23 axis. Mol Med Rep 25:N/A (2022). PubMed: 35475447
Risteski P et al. Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment. Cell Rep 40:111169 (2022). PubMed: 35926461
Boikova A et al. HRas and Myc synergistically induce cell cycle progression and apoptosis of murine cardiomyocytes. Front Cardiovasc Med 9:948281 (2022). PubMed: 36337898
Halcrow EFJ et al. Midbody Proteins Display Distinct Dynamics during Cytokinesis. Cells 11:N/A (2022). PubMed: 36359734
Pallavicini G et al. Goldberg-Shprintzen syndrome protein KIF1BP is a CITK interactor implicated in cytokinesis. J Cell Sci 134:N/A (2021). PubMed: 34100550
Yao DW et al. Kinesin family member 23 (KIF23) contributes to the progression of bladder cancer cells in vitro and in vivo. Neoplasma 68:298-306 (2021). PubMed: 33231086
Li T et al. Methylation-mediated repression of MiR-424/503 cluster promotes proliferation and migration of ovarian cancer cells through targeting the hub gene KIF23. Cell Cycle 18:1601-1618 (2019). PubMed: 31135262
S Pedersen R et al. Profiling DNA damage response following mitotic perturbations. Nat Commun 7:13887 (2016). PubMed: 27976684

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (9)

レビューと Q&A