Anti-MKK6 抗体 (ab154901)
Key features and details
- Rabbit polyclonal to MKK6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MKK6 antibody
MKK6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MKK6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow
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免疫原
Recombinant fragment corresponding to Human MKK6 aa 1-334.
Database link: P52564 -
ポジティブ･コントロール
- WB: HEK-293T whole cell lysate. ICC/IF: HeLa cells. IHC-P: Human N87 xenograft tissue, mouse duodenum tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154901の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively. -
組織特異性
Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Weakly autophosphorylated.
Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 286883 Cow
- Entrez Gene: 5608 Human
- Entrez Gene: 26399 Mouse
- Entrez Gene: 114495 Rat
- Omim: 601254 Human
- SwissProt: Q5E9X2 Cow
- SwissProt: P52564 Human
- SwissProt: P70236 Mouse
see all -
製品の状態
It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules. -
別名
- Dual specificity mitogen activated protein kinase kinase 6 antibody
- Dual specificity mitogen-activated protein kinase kinase 6 antibody
- MAP kinase kinase 6 antibody
see all
画像
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Western blot - Anti-MKK6 antibody (ab154901)Anti-MKK6 antibody (ab154901) at 1/1000 dilution + HEK-293T whole cell lysate at 30 µg
Predicted band size: 37 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-MKK6 antibody (ab154901)Immunofluorescent analysis of methanol-fixed HeLa cells labeling MKK6 with ab154901 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKK6 antibody (ab154901)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human N87 xenograft tissue labeling MKK6 with ab154901 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MKK6 antibody (ab154901)Paraffin embedded mouse duodenum tissue stained for MEK6 using ab154901 at 1/500 dilution in immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab154901 は 3 報の論文で使用されています。
- Huang J et al. Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway. Phytother Res 37:731-742 (2023). PubMed: 36196887
- Wu G et al. CircASPH promotes KGN cells proliferation through miR-375/MAP2K6 axis in Polycystic Ovary Syndrome. J Cell Mol Med 26:1817-1825 (2022). PubMed: 33372369
- Tang B et al. PEA15 promotes liver metastasis of colorectal cancer by upregulating the ERK/MAPK signaling pathway. Oncol Rep 41:43-56 (2019). PubMed: 30365128