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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-MK2 抗体 [E341] (ab32567)

Reviews (1) Submit a question References (3)
Western blot - Anti-MK2 antibody [E341] (ab32567)
  • Western blot - Anti-MK2 antibody [E341] (ab32567)
  • Western blot - Anti-MK2 antibody [E341] (ab32567)
  • Anti-MK2 antibody [E341] (ab32567)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E341] to MK2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MK2 antibody [E341]
    MK2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [E341] to MK2

  • 由来種

    Rabbit

  • 特異性

    ab32567 recognises MAPKAP Kinase-2.

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF or IHC

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human MK2 aa 350-450 (C terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32567の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
特記事項
WB
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC.

ターゲット情報

  • 機能

    Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.

  • 組織特異性

    Expressed in all tissues examined.

  • 配列類似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylated and activated by MAP kinase.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • MAP kinase activated protein Kinase 2 antibody
    • MAP kinase-activated protein kinase 2 antibody
    • MAPK activated protein kinase 2 antibody
    • MAPK-activated protein kinase 2 antibody
    • MAPK2_HUMAN antibody
    • MAPKAP K2 antibody
    • MAPKAP kinase 2 antibody
    • MAPKAPK 2 antibody
    • MAPKAPK-2 antibody
    • MAPKAPK2 antibody
    • Mitogen activated protein kinase activated protein kinase 2 antibody
    • MK 2 antibody
    • MK2 antibody
    see all

画像

  • Western blot - Anti-MK2 antibody [E341] (ab32567)
    Western blot - Anti-MK2 antibody [E341] (ab32567)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-MK2 antibody [E341] (ab32567)
    Western blot - Anti-MK2 antibody [E341] (ab32567)
    Anti-MK2 antibody [E341] (ab32567) at 1/1000 dilution + HeLa cell lysate

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Western blot - Anti-MK2 antibody [E341] (ab32567)
    Western blot - Anti-MK2 antibody [E341] (ab32567)
    Lanes 1-2 : Anti-MK2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution
    Lanes 3-4 : Anti-MK2 antibody [E341] (ab32567) at 1/500 dilution

    Lanes 1 & 3 : UV treated HeLa cells
    Lanes 2 & 4 : UV treated HeLa cells + p38 inhibitor

    Secondary
    All lanes : IRDye® 680 LICOR goat anti-rabbit.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?
    Additional bands at: 55 kDa (possible cross reactivity)


    Exposure time: 1 minute


    ab32567 recognise total MK2 (lane 3,4). A control for specificity is shown as UV treated HeLa + p38 inhibitor (lane 2,4). While the intensity of pT334 band is greatly reduced (lane 2), total MK2 remains unaltered (lane 4).

    See Abreview

  • Anti-MK2 antibody [E341] (ab32567)
    Anti-MK2 antibody [E341] (ab32567)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab32567 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32567 は 3 報の論文で使用されています。

  • Xuan W  et al. Propofol Protects Against Erastin-Induced Ferroptosis in HT-22 Cells. J Mol Neurosci 72:1797-1808 (2022). PubMed: 35727524
  • Muraleva NA  et al. SkQ1 Suppresses the p38 MAPK Signaling Pathway Involved in Alzheimer's Disease-Like Pathology in OXYS Rats. Antioxidants (Basel) 9:N/A (2020). PubMed: 32731533
  • McCaskill JL  et al. Broad-Spectrum Inhibition of Respiratory Virus Infection by MicroRNA Mimics Targeting p38 MAPK Signaling. Mol Ther Nucleic Acids 7:256-266 (2017). Dog, Human . PubMed: 28624201

レビューと Q&A

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Western blot abreview for Anti-MK2 antibody [E341]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
35 µg
Treatment
UV treated samples (20 J dose)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Aug 20 2012

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