JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB8956

Anti-Mitotic Cells 抗体 [8B3G]

Anti-Mitotic Cells antibody [8B3G]

2

(2 Reviews)

|

(2 Publications)

Mouse Monoclonal Mitotic Cells antibody. Suitable for IHC-Fr, Flow Cyt and reacts with Zebrafish, Human samples. Cited in 2 publications.
3 Images
Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (AB8956)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (AB8956)

Dual parameter flow cytometric analysis of human colon cancer HT29 cells stained with monoclonal antibody 8B3G and propidium iodide (PI). The mitotic cell fraction is encircled.

Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956)

ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone : Methanol 1 : 1. ab8956 used at 1 : 50 dilution and incubated for 45 minutes at room temperature.

Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956)

ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone : Methanol 1 : 1. ab8956 used at 1 : 50 dilution and incubated for 45 minutes at room temperature.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

8B3G

アイソタイプ

IgM

キャリアフリー

No

交差種

Human, Zebrafish

アプリケーション

Flow Cyt, IHC-Fr

applications

特異性

Strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) this antibody clearly distinguishes these M-phase cells from cell at other stages of the cell cycle. Dynamic information can be obtained by combining BrdU incorporation with antibody staining, which can distinguish and quantitate the four major fractions of the cell cycle. This antibody can be used for flow cytometric analyses and immunocytochemistry, it is not suitable for immunoblotting.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/100", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.</p>" }, "Zebrafish": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
Preservative: 0.09% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 12:308-319 PubMed30453269

2018

Phosphoproteome Profiling Reveals Multifunctional Protein NPM1 as part of the Irradiation Response of Tumor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine Wiesmann,Rita Gieringer,Franz Grus,Juergen Brieger

The American journal of pathology 168:878-87 PubMed16507903

2006

Cell-cycle markers in a transgenic mouse model of human tauopathy: increased levels of cyclin-dependent kinase inhibitors p21Cip1 and p27Kip1.

Applications

Unspecified application

Species

Mouse

Patrice Delobel,Isabelle Lavenir,Bernardino Ghetti,Max Holzer,Michel Goedert
View all publications

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com