AB12039

Anti-MiTF 抗体 [C5]

Name: Anti-MiTF 抗体 [C5] (ab12039) | Abcam
Brand: Abcam Limited
SKU: AB12039
Price: 75000 JPY
Availability: InStock
Rating: 4 (5 reviews)

Anti-MiTF antibody [C5]

(5 Reviews)

(128 Publications)

Anti-MiTF antibody [C5] (ab12039) is a mouse monoclonal antibody detecting MiTF in Western Blot, Flow Cytometry (Intra), ICC/IF. Suitable for Human.

- Over 110 publications
- Trusted since 2004

別名を表示する

BHLHE32, MITF, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32

3 Images

Project

Western blot - Anti-MiTF antibody [C5] (AB12039)

All lanes:

Western blot - Anti-MiTF antibody [C5] (ab12039) at 1 µg/mL

All lanes:

Malme 3m (Human melanoma cells) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 58 kDa

Observed band size: 40 kDa,55 kDa,70 kDa

true

Exposure time: 20min

Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (AB12039)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (AB12039)

Overlay histogram showing Malme 3m cells stained with ab12039 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12039, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (AB12039)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (AB12039)

ab12039 staining MiTF in Malme-3M cells. The cells were permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab12039 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-MiTF antibody [C5] (ab12039) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of MiTF?
Anti-MiTF [C5] (ab12039) specifically detects a band for MiTF (UniProt: O75030) at a molecular weight of 59kDa.

Trusted by the scientific community
Anti-MiTF [C5] (ab12039) was first used in a scientific publication in 2004 and has been cited over 110 times in peer-reviewed journals.

Reviewed by scientists
Anti-MiTF [C5] (ab12039) has over 5 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [C5] also available for your convenience: ab12039, Carrier free - ab264110

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Involved in the cellular response to amino acid availability by acting downstream of MTOR : in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation (PubMed : 36608670). Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity (PubMed : 36608670). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758).

See full target information MITF

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文献 (128)

Recent publications for all applications. Explore the full list and refine your search

Nature biotechnology : PubMed41028831

2025

Derivation of embryonic stem cells across avian species.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Chen,Zheng Guo,Xinyi Tong,Xizi Wang,Xugeng Liu,Hiroki Nagai,Ping Wu,Jiayi Lu,David Huss,Martin Tran,Carol Readhead,Christina Wu,Lin Cao,Yixin Huang,Zhaohan Zeng,Fan Feng,Nima Adhami,Sirjan Mor,Rusty Lansford,Cheng-Ming Chuong,Guojun Sheng,Carlos Lois,Qi-Long Ying

PLoS genetics 21:e1011693 PubMed40354322

2025

Molecular insights into region-specific sexual dichromatism: Comparative transcriptome analysis of red cheek pigmentation in zebra finches.

Applications

Unspecified application

Species

Unspecified reactive species

Gee-Way Lin,Chih-Kuan Chen,Ting-Xin Jiang,Ya-Chen Liang,Pin-Chi Tang,Ping Wu,Randall B Widelitz,Chih-Feng Chen,Cheng-Ming Chuong

The Journal of clinical investigation 135: PubMed40311102

2025

Tebentafusp elicits on-target cutaneous immune responses driven by cytotoxic T cells in uveal melanoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Ramon Staeger,Aizhan Tastanova,Adhideb Ghosh,Nicola Winkelbeiner,Prachi Shukla,Isabel Kolm,Patrick Turko,Adel Benlahrech,Jane Harper,Anna Broomfield,Antonio Camera,Marianna Ambrosio,Veronika Haunerdinger,Phil F Cheng,Egle Ramelyte,James Pham,Stefanie Kreutmair,Burkhard Becher,Mitchell P Levesque,Reinhard Dummer,Barbara Meier-Schiesser

Regenerative therapy 26:749-759 PubMed39290629

2024

Efficient and cost-effective differentiation of induced neural crest cells from induced pluripotent stem cells using laminin 211.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuma Takahashi,Shizuka Aritomi,Fumie Honkawa,Sayaka Asari,Ken Hirose,Atsushi Konishi

EMBO reports 25:4252-4280 PubMed39169200

2024

Novel mechanisms of MITF regulation identified in a mouse suppressor screen.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Nhung Vu,Matti Már Valdimarsson,Sara Sigurbjörnsdóttir,Kristín Bergsteinsdóttir,Julien Debbache,Keren Bismuth,Deborah A Swing,Jón H Hallsson,Lionel Larue,Heinz Arnheiter,Neal G Copeland,Nancy A Jenkins,Petur O Heidarsson,Eiríkur Steingrímsson

Cell reports 43:114484 PubMed38990725

2024

Feedforward cysteine regulation maintains melanoma differentiation state and limits metastatic spread.

Applications

Unspecified application

Species

Unspecified reactive species

Deyang Yu,Jiaxin Liang,Hans R Widlund,Pere Puigserver

International journal of biological sciences 20:1688-1704 PubMed38481807

2024

Interruption of p38-MSK1-CREB-MITF-M pathway to prevent hyperpigmentation in the skin.

Applications

Unspecified application

Species

Unspecified reactive species

Song-Hee Kim,Jiyeon Lee,Jihye Jung,Ga Hyun Kim,Cheong-Yong Yun,Sang-Hun Jung,Bang Yeon Hwang,Jin Tae Hong,Sang-Bae Han,Jae-Kyung Jung,Youngsoo Kim

Cell death & disease 15:208 PubMed38472212

2024

The MITF/mir-579-3p regulatory axis dictates BRAF-mutated melanoma cell fate in response to MAPK inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Domenico Liguoro,Rachele Frigerio,Arianna Ortolano,Andrea Sacconi,Mario Acunzo,Giulia Romano,Giovanni Nigita,Barbara Bellei,Gabriele Madonna,Mariaelena Capone,Paolo Antonio Ascierto,Rita Mancini,Gennaro Ciliberto,Luigi Fattore

International journal of biological sciences 20:312-330 PubMed38164184

2024

Targeting phosphorylation circuits on CREB and CRTCs as the strategy to prevent acquired skin hyperpigmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Song-Hee Kim,Changseon Na,Cheng-Yong Yun,Jun Gu Kim,Seung Tae Baek,Hyun Jin An,Jae Duk Lee,Seung Wha Lee,Jae-Kyung Jung,Bang Yeon Hwang,Sang-Bae Han,Youngoo Kim

Frontiers in cell and developmental biology 11:1252547 PubMed37691820

2023

Integration of human stem cell-derived systems and mouse preclinical models identifies complex pathophysiologic mechanisms in retinal dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa K Jones,Luz D Orozco,Han Qin,Tom Truong,Patrick Caplazi,Justin Elstrott,Zora Modrusan,Shawnta Y Chaney,Marion Jeanne

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MiTF 抗体 [C5]

Anti-MiTF antibody [C5]

Western blot - Anti-MiTF antibody [C5] (AB12039)

Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (AB12039)

Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (AB12039)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (128)

Abcam product promise