Anti-MIST-1 抗体 (ab187978)
Key features and details
- Rabbit polyclonal to MIST-1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-MIST-1 antibody
MIST-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MIST-1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human MIST-1 aa 100 to the C-terminus.
Database link: Q7RTS1 -
ポジティブ･コントロール
- IHC-P: Human pancreas, stomach, tonsil and skeletal muscle tissue; ICC/IF: HepG2 whole cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187978の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| 特記事項 |
|---|
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
ターゲット情報
-
機能
Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3'. -
組織特異性
Expressed in brain, liver, spleen and skeletal muscle. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
ドメイン
Lacks a classic transcription activation domain and instead possesses an N-terminal region capable of inhibiting heterologous activators. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 168620 Human
- Omim: 608606 Human
- SwissProt: Q7RTS1 Human
- Unigene: 674510 Human
-
別名
- Basic helix loop helix domain containing class B 8 antibody
- Basic helix loop helix family member a15 antibody
- BHA15_HUMAN antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling MIST-1 in the nucleus of exocrine glandular cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunocytochemistry/ Immunofluorescence - Anti-MIST-1 antibody (ab187978)Immuncytochemical analysis of HepG2 (Human liver hepatocellular carcinoma cell line) whole cells labeling MIST-1 in the nucleoplasm and the Golgi apparatus with ab187978 at 2 µg/ml.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MIST-1 in the nucleus of non-germinal center cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling MIST-1 in the nucleus of glandular cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling MIST-1 in the striated muscle fiber (no positivity) with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (11)
ab187978 は 11 報の論文で使用されています。
- Chatzeli L et al. A cellular hierarchy of Notch and Kras signaling controls cell fate specification in the developing mouse salivary gland. Dev Cell 58:94-109.e6 (2023). PubMed: 36693323
- Wahl AM et al. Structural and functional analysis of salivary intercalated duct cells reveals a secretory phenotype. J Physiol 601:4539-4556 (2023). PubMed: 37724716
- Bordeu I et al. Inflationary theory of branching morphogenesis in the mouse salivary gland. Nat Commun 14:3422 (2023). PubMed: 37296120
- Uchida H et al. Short-term and bystander effects of radiation on murine submandibular glands. Dis Model Mech 15:N/A (2022). PubMed: 36263624
- Yoon YJ et al. Salivary gland organoid culture maintains distinct glandular properties of murine and human major salivary glands. Nat Commun 13:3291 (2022). PubMed: 35672412