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リコンビナントRabMAb

Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 抗体 [EPR19900-275] - BSA and Azide free (ab245831)

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Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)
  • Immunocytochemistry/ Immunofluorescence - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)
  • Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)
  • Flow Cytometry (Intracellular) - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)
  • Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19900-275] to MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Unconjugated

製品の概要

  • 製品名

    Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free

  • 製品の詳細

    Rabbit monoclonal [EPR19900-275] to MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: THP-1 cells (treated with 80nM TPA overnight, then treated with 100ng/ml LPS for 3h and together with 300ng/ml BFA for another 3h). Flow Cyt (intra): THP-1 cells (treated with 80nM TPA overnight, then treated with 100ng/ml LPS for 3h and together with 300ng/ml BFA for another 3h).

  • 特記事項

    ab245831 is the carrier-free version of ab206429.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245831の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
ICC/IF
Use at an assay dependent concentration.
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

画像

  • Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)
    Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)
    All lanes : Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] (ab206429) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 2 : THP-1 (1 treated with 80nM TPA overnight, then 100 ng/ml LPS for 3 hours and then 300 ng/ml Brefeldin A was added for the last 3 hours) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 10 kDa
    Observed band size: 12 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab206429, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)
    Immunocytochemistry/ Immunofluorescence - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)

    This data was developed using ab206429, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab206429 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in THP-1 cells treated with TPA (80nM overnight), then treated with 100ng/ml LPS for 3h and together with 300ng/ml BFA for another 3h. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)
    Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)
    All lanes : Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] (ab206429) at 1/1000 dilution

    Lane 1 : His-tagged mouse CCL3 Recombinant Protein
    Lane 2 : His-tagged human CCL3 Recombinant Protein
    Lane 3 : His-tagged human CCL4 Recombinant Protein
    Lanes 4-5 : His-tagged human CCL18 Recombinant Protein
    Lane 6 : GST-tagged human CCL3L1 Recombinant Protein
    Lane 7 : GST-tagged human CCL4L1 Recombinant Protein

    Predicted band size: 10 kDa



    Exposure time:
    Lanes 1-7 (left image): 180 seconds
    Lane 6 (right image): 5 seconds

    This data was developed using ab206429, the same antibody clone in a different buffer formulation.

     

  • Flow Cytometry (Intracellular) - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)
    Flow Cytometry (Intracellular) - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)

    This data was developed using ab206429, the same antibody clone in a different buffer formulation. 

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 0.1% Tween 20 permeabilized THP-1 (human monocytic leukemia cell line) (treated with 80nM TPA overnight, then treated with 100ng/ml LPS for 3h and together with 300ng/ml BFA for another 3 hr) (red) or untreated (green) cells labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab206429 at 1/600 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)
    Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA and Azide free (ab245831)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab245831 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab245831 は論文での使用が確認できていません。

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