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AB242545

Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta 抗体 [EPR19900-275] - BSA and Azide free (Capture)

Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture)

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Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture) (ab242545) is a rabbit recombinant monoclonal antibody in a PBS only buffer for easy conjugation detecting CCL3L1 in ELISA. Suitable for Human, Monkey.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

G0S19-1, MIP1A, SCYA3, CCL3, C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, MIP-1-alpha

1 Images
Sandwich ELISA - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture) (AB242545)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture) (AB242545)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab214569).

関連する標識済み抗体及び組成の異なる製品 (4)

  • Carrier free

    Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA & Azide free

  • Unconjugated

    Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR19900-275

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human, Monkey

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Monkey": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab242545 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human MIP1a. The recommended pair for sandwich ELISA is:
Capture: ab242545, Human MIP1a Capture Antibody (unconjugated)
Detector: ab242793, Human MIP1a Detector Antibody (unconjugated)
The reference range value is 0.14 - 100 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 and MIP-1 beta known as CCL4 are key chemokines involved in the immune response. These proteins have a molecular mass of approximately 8 to 10 kDa. They are secreted by various cells including macrophages dendritic cells and lymphocytes. MIP-1 alpha and MIP-1 beta are part of the CC chemokine family and are secreted as monomers that bind to specific receptors on the surface of target cells attracting and activating them during inflammation and immune responses.
Biological function summary

These chemokines play significant roles in the recruitment and activation of leukocytes to sites of infection or injury. They are important elements in the immune defense system and help to orchestrate the movement and coordination of immune cells. MIP-1 alpha and MIP-1 beta function in synergy with other chemokines and cytokines enhancing the body's ability to respond to pathogens. While they do not form part of a larger complex their interaction with chemokine receptors like CCR1 CCR4 and CCR5 is critical for their function.

Pathways

MIP-1 alpha and MIP-1 beta participate in inflammatory and immune pathways. They are key mediators in the chemokine signaling pathway linked especially with the activation of the MAPK and NF-kB pathways. These chemokines work closely with related chemokines such as CCL5 (RANTES) and cytokines like TNF-alpha amplifying the body's response to immune challenges.

MIP-1 alpha and MIP-1 beta have connections to several pathological conditions particularly inflammatory diseases and HIV. Their levels often increase in diseases like rheumatoid arthritis reflecting their role in inflammatory processes. In the context of HIV infection these chemokines inhibit viral entry by blocking the CCR5 receptor which is important for some strains of the virus. This highlights their therapeutic potential in designing treatments targeting HIV alongside other inflammatory conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
See full target information CCL3

追加のターゲット

CCL4
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組合せ製品

Assay Kits

AB229400

Human MIP-1 alpha/CCL3 ELISA Kit, Fluorescent

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Sandwich ELISA - Human MIP-1 alpha/CCL3 ELISA Kit, Fluorescent (AB229400)

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Primary Antibodies

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Anti-GM-CSF antibody [EPR22272-71] - BSA and Azide free (Detector)

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Sandwich ELISA - Anti-GM-CSF antibody [EPR22272-71] - BSA and Azide free (Detector) (AB252634)

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  • 0
Primary Antibodies

AB278327

Anti-CCL1 antibody [EPR24171-79] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CCL1 antibody [EPR24171-79] - BSA and Azide free (Detector) (AB278327)

  • 0
  • 0
Primary Antibodies

AB290643

Anti-CXCL9 antibody [EPR26512-118]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL9 antibody [EPR26512-118] (AB290643)

  • 0
  • 0
Primary Antibodies

AB302730

Anti-CXCL16 antibody [EPR25370-28] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CXCL16 antibody [EPR25370-28] - BSA and Azide free (Detector) (AB302730)

  • 0
  • 0

Abcam product promise

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