AB17787

Anti-Milk Fat Globule 1 抗体 [SPM291]

Name: Anti-Milk Fat Globule 1 抗体 [SPM291] (ab17787) | Abcam
Brand: Abcam Limited
SKU: AB17787
Rating: 3 (1 reviews)

Anti-Milk Fat Globule 1 antibody [SPM291]

BOND RX™ Validated
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(1 Review)

(6 Publications)

Mouse Monoclonal Milk Fat Globule 1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications.

別名を表示する

Lactadherin, Breast epithelial antigen BA46, HMFG, MFGM, Milk fat globule-EGF factor 8, SED1, MFG-E8, MFGE8

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

IHC image of ab17787 staining in human carinoma breast formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Milk Fat Globule 1 with ab18878 at 1 ug/ml.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

IHC image of ab17787 staining in human carinoma Renal Cell formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling Milk Fat Globule 1 with ab18878 at 1 ug/ml.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

SPM291

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.09% Sodium azide Constituents: BSA, 0.0268% PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.. Medin. Main constituent of aortic medial amyloid.

See full target information MFGE8

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 145: PubMed30093551

2018

Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self-amplifying radial glial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marybeth Baumgartner,Anouk M Olthof,Gabriela S Aquino,Katery C Hyatt,Christopher Lemoine,Kyle Drake,Nikita Sturrock,Nhut Nguyen,Sahar Al Seesi,Rahul N Kanadia

Nature communications 9:3133 PubMed30087345

2018

Regulation of striatal cells and goal-directed behavior by cerebellar outputs.

Applications

Unspecified application

Species

Unspecified reactive species

Le Xiao,Caroline Bornmann,Laetitia Hatstatt-Burklé,Peter Scheiffele

Reproduction (Cambridge, England) 152:225-33 PubMed27340235

2016

MFGE8 regulates TGF-β-induced epithelial mesenchymal transition in endometrial epithelial cells in vitro.

Applications

ICC/IF, WB

Species

Human, Human

Liang Yu,Rong Hu,Claretta Sullivan,R James Swanson,Sergio Oehninger,Ying-Pu Sun,Silvina Bocca

The Journal of biological chemistry 289:24560-72 PubMed25006249

2014

Polarization of prostate cancer-associated macrophages is induced by milk fat globule-EGF factor 8 (MFG-E8)-mediated efferocytosis.

Applications

IHC-P

Species

Human

Fabiana N Soki,Amy J Koh,Jacqueline D Jones,Yeo Won Kim,Jinlu Dai,Evan T Keller,Kenneth J Pienta,Kamran Atabai,Hernan Roca,Laurie K McCauley

Fertility and sterility 101:552-9 PubMed24262600

2013

Tumor necrosis factor α up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor κB activation, resulting in cell migration of epithelial cells.

Applications

Unspecified application

Species

Human

Liang Yu,Sandra Anderson,Sergio Oehninger,Silvina Bocca

Molecular human reproduction 17:360-71 PubMed21177637

2010

Expression of milk fat globule EGF-factor 8 (MFG-E8) mRNA and protein in the human endometrium and its regulation by prolactin.

Applications

WB, IHC-Fr, IHC-P, ICC/IF

Species

Human, Human, Human, Human

Anahi Franchi,Silvina Bocca,Sandra Anderson,Ryan Riggs,Sergio Oehninger

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Milk Fat Globule 1 抗体 [SPM291]

Anti-Milk Fat Globule 1 antibody [SPM291]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (6)

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