Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-MIF 抗体 [EPR18149-128] (ab187064)

Submit a review Submit a question References (5)
Western blot - Anti-MIF antibody [EPR18149-128] (ab187064)
  • Western blot - Anti-MIF antibody [EPR18149-128] (ab187064)
  • Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-128] (ab187064)
  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR18149-128] (ab187064)
  • Western blot - Anti-MIF antibody [EPR18149-128] (ab187064)
  • Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-128] (ab187064)
  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR18149-128] (ab187064)
  • Anti-MIF antibody [EPR18149-128] (ab187064)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18149-128] to MIF
  • Suitable for: ICC/IF, WB, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MIF antibody [EPR18149-128]
    MIF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR18149-128] to MIF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human fetal brain and fetal spleen lysates; Mouse brain and kidney lysate; Rat brain lysate; J774A.1, Y79, Jurkat, NIH/3T3, RAW 264.7, C6 and Neuro-2a whole cell lysates. ICC/IF: Neuro-2a and RAW 264.7 cells. Flow Cyt (intra): Neuro-2a and RAW 264.7 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR18149-128

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187064の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
Flow Cyt (Intra)
1/500.
特記事項
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
Flow Cyt (Intra)
1/500.

ターゲット情報

  • 機能

    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

  • 関連疾患

    Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.

  • 配列類似性

    Belongs to the MIF family.

  • 細胞内局在

    Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • GIF antibody
    • GLIF antibody
    • Glycosylation inhibiting factor antibody
    • Glycosylation-inhibiting factor antibody
    • L-dopachrome isomerase antibody
    • L-dopachrome tautomerase antibody
    • Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
    • Macrophage migration inhibitory factor antibody
    • MIF antibody
    • MIF protein antibody
    • MIF_HUMAN antibody
    • MMIF antibody
    • Phenylpyruvate tautomerase antibody
    see all

画像

  • Western blot - Anti-MIF antibody [EPR18149-128] (ab187064)
    Western blot - Anti-MIF antibody [EPR18149-128] (ab187064)
    All lanes : Anti-MIF antibody [EPR18149-128] (ab187064) at 1/1000 dilution

    Lane 1 : Wild type HAP1 whole cell lysate
    Lane 2 : MIF knockout HAP1 whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
    Lane 4 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Lysates/proteins at 20 µg/ml per lane.

    Predicted band size: 13 kDa
    Observed band size: 13 kDa


    Exposure time: 15 seconds


    Ab187064 was shown to specifically react with MIF in wild-type HAP1 cells as signal was lost in MIF knockout cells. Wild-type and MIF knockout samples were subjected to SDS-PAGE. ab187064 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

  • Western blot - Anti-MIF antibody [EPR18149-128] (ab187064)
    Western blot - Anti-MIF antibody [EPR18149-128] (ab187064)
    All lanes : Anti-MIF antibody [EPR18149-128] (ab187064) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal spleen lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate
    Lane 5 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage cell line) whole cell lysate
    Lane 6 : Mouse kidney lysate
    Lane 7 : Y79 (human retinoblastoma cell line) whole cell lysate
    Lane 8 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 9 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 & 7-8 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
    Lanes 3-6 & 9 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 13 kDa
    Observed band size: 13 kDa



     

    Exposure time : Lanes 1,2,3 and 4: 10 seconds; Lanes 5 and 6: 3 seconds; Lanes 7,8 and 9: 1 second.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-128] (ab187064)
    Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-128] (ab187064)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labeling MIF with ab187064 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Neuro-2a cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR18149-128] (ab187064)
    Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR18149-128] (ab187064)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Neuro-2a (mouse neuroblastoma cell line) cell line labeling MIF with ab187064 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Western blot - Anti-MIF antibody [EPR18149-128] (ab187064)
    Western blot - Anti-MIF antibody [EPR18149-128] (ab187064)
    All lanes : Anti-MIF antibody [EPR18149-128] (ab187064) at 1/5000 dilution

    Lane 1 : NIH/3T3 (mouseembryo fibroblast cell line) whole cell lysate
    Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : C6 (rat glial tumor cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 13 kDa
    Observed band size: 13 kDa



     

    Exposure time : Lane 1: 5 seconds; Lane 2: 1 second; Lane 3: 30 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-128] (ab187064)
    Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-128] (ab187064)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling MIF with ab187064 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on RAW 264.7 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR18149-128] (ab187064)
    Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR18149-128] (ab187064)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling MIF with ab187064 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Anti-MIF antibody [EPR18149-128] (ab187064)
    Anti-MIF antibody [EPR18149-128] (ab187064)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (5)

ab187064 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab187064 は 5 報の論文で使用されています。

  • Huang Y  et al. A pref-1-controlled non-inflammatory mechanism of insulin resistance. iScience 26:106923 (2023). PubMed: 37283810
  • Zhang Y  et al. Macrophage migration inhibitory factor (MIF) promotes intervertebral disc degeneration through the NF-κB pathway, and the MIF inhibitor CPSI-1306 alleviates intervertebral disc degeneration in a mouse model. FASEB J 37:e23303 (2023). PubMed: 37983963
  • Cui N  et al. Exercise inhibits JNK pathway activation and lipotoxicity via macrophage migration inhibitory factor in nonalcoholic fatty liver disease. Front Endocrinol (Lausanne) 13:961231 (2022). PubMed: 36147562
  • Nakahara K  et al. Attenuation of Macrophage Migration Inhibitory Factor-Stimulated Signaling via S-Nitrosylation. Biol Pharm Bull 42:1044-1047 (2019). PubMed: 31155581
  • Li XJ  et al. Proteomics of Uterosacral Ligament Connective Tissue from Women with and without Pelvic Organ Prolapse. Proteomics Clin Appl 13:e1800086 (2019). PubMed: 30516354

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab187064.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com