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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-MIF 抗体 [EPR12463] (ab175189)

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Western blot - Anti-MIF antibody [EPR12463] (ab175189)
  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)
  • Western blot - Anti-MIF antibody [EPR12463] (ab175189)
  • Western blot - Anti-MIF antibody [EPR12463] (ab175189)
  • Western blot - Anti-MIF antibody [EPR12463] (ab175189)
  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)
  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)
  • Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)
  • Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)
  • Anti-MIF antibody [EPR12463] (ab175189)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12463] to MIF
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

製品の概要

  • 製品名

    Anti-MIF antibody [EPR12463]
    MIF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12463] to MIF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IPmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human MIF aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P14174

  • ポジティブ･コントロール

    • WB: Y79, THP-1, and Molt-4 cell lysates. Mouse and rat brain tissue lysates. Flow Cyt (intra): THP-1, and Molt-4 cells. IP: Human fetal brain tissue lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR12463

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab175189の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/140.

For unpurified use at 1/50.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 12 kDa.

For unpurified use at 1/500 - 1/1000.
IP
1/50.

For unpurified use at 1/30.
特記事項
Flow Cyt (Intra)
1/140.

For unpurified use at 1/50.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 12 kDa.

For unpurified use at 1/500 - 1/1000.
IP
1/50.

For unpurified use at 1/30.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

  • 関連疾患

    Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.

  • 配列類似性

    Belongs to the MIF family.

  • 細胞内局在

    Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • GIF antibody
    • GLIF antibody
    • Glycosylation inhibiting factor antibody
    • Glycosylation-inhibiting factor antibody
    • L-dopachrome isomerase antibody
    • L-dopachrome tautomerase antibody
    • Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
    • Macrophage migration inhibitory factor antibody
    • MIF antibody
    • MIF protein antibody
    • MIF_HUMAN antibody
    • MMIF antibody
    • Phenylpyruvate tautomerase antibody
    see all

画像

  • Western blot - Anti-MIF antibody [EPR12463] (ab175189)
    Western blot - Anti-MIF antibody [EPR12463] (ab175189)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: MIF knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab175189 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab175189 was shown to specifically react with MIF in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when knockout cells were examined. Wild-type and MIF knockout samples were subjected to SDS-PAGE. Ab175189 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/2,0000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)
    Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)

    Intracellular Flow Cytometry analysis of THP-1 cells labelling MIF with unpurified ab175189 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary anitbody. A rabbit monoclonal IgG was used as the isotype control (green). 

     

     

  • Western blot - Anti-MIF antibody [EPR12463] (ab175189)
    Western blot - Anti-MIF antibody [EPR12463] (ab175189)
    All lanes : Anti-MIF antibody [EPR12463] (ab175189) at 1/1000 dilution (unpurified)

    Lane 1 : Y79 cell lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate

    Lysates/proteins at 20 µg/ml per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-MIF antibody [EPR12463] (ab175189)
    Western blot - Anti-MIF antibody [EPR12463] (ab175189)
    All lanes : Anti-MIF antibody [EPR12463] (ab175189) at 1/2000 dilution (purified)

    Lane 1 : Y79 cell lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-MIF antibody [EPR12463] (ab175189)
    Western blot - Anti-MIF antibody [EPR12463] (ab175189)
    All lanes : Anti-MIF antibody [EPR12463] (ab175189) at 1/1000 dilution (unpurified)

    Lane 1 : Y79 lysates
    Lane 2 : THP-1 lysates
    Lane 3 : Molt-4 lysates
    Lane 4 : Human fetal brain lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 12 kDa

  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)
    Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)

    Intracellular Flow Cytometry analysis of THP-1 cells labelling MIF with purified ab175189 at 1/140 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary anitbody. A rabbit monoclonal IgG was used as the isotype control (green). 

     

     

  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)
    Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)

    Intracellular flow cytometric analysis of permeabilized Molt-4 cells using unpurified ab175189 at a 1/10 dilution (red) or a rabbit IgG (negative) (green). 

     

     

  • Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)
    Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)

    ab175189 (unpurified) at 1/30 immunoprecipitating MIF in human fetal brain. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)
    Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)

    ab175189 (purified) at 1/50 immunoprecipitating MIF in human fetal brain. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-MIF antibody [EPR12463] (ab175189)
    Anti-MIF antibody [EPR12463] (ab175189)

参考文献 (12)

ab175189 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab175189 は 12 報の論文で使用されています。

  • Becker-Herman S  et al. CD74 is a regulator of hematopoietic stem cell maintenance. PLoS Biol 19:e3001121 (2021). PubMed: 33661886
  • Luo Q  et al. OTUD1 Activates Caspase-Independent and Caspase-Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 Degradation. Adv Sci (Weinh) 8:2002874 (2021). PubMed: 33898171
  • Kurelac I  et al. Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses. Nat Commun 10:903 (2019). PubMed: 30796225
  • De R  et al. Macrophage migration inhibitory factor regulates mitochondrial dynamics and cell growth of human cancer cell lines through CD74-NF-?B signaling. J Biol Chem 293:19740-19760 (2018). PubMed: 30366984
  • Li J  et al. Macrophage migration inhibitory factor promotes Warburg effect via activation of the NF-?B/HIF-1a pathway in lung cancer. Int J Mol Med 41:1062-1068 (2018). WB . PubMed: 29207023
View all Publications for this product

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