Anti-MIF 抗体 (ab7207)
Key features and details
- Rabbit polyclonal to MIF
- Suitable for: WB, IHC-Fr, ICC/IF, ELISA, IP, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-MIF antibody
MIF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MIF -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Fr, ICC/IF, ELISA, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant full length protein corresponding to Rat MIF.
Database link: P30904 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7207の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
|
| IHC-Fr | (3) |
Use at an assay dependent concentration.
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| ELISA | (1) |
Use at an assay dependent concentration. PubMed: 19066630
|
| IP |
Use at an assay dependent concentration.
|
|
| IHC-P | (2) |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. PubMed: 19066630 |
|
IP
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. -
関連疾患
Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. -
配列類似性
Belongs to the MIF family. -
細胞内局在
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. - Information by UniProt
-
参照データベース
- Entrez Gene: 17319 Mouse
- Entrez Gene: 81683 Rat
- SwissProt: P34884 Mouse
- SwissProt: P30904 Rat
- Unigene: 2326 Mouse
- Unigene: 2661 Rat
-
別名
- GIF antibody
- GLIF antibody
- Glycosylation inhibiting factor antibody
see all
画像
-
Western blot - Anti-MIF antibody (ab7207)Anti-MIF antibody (ab7207) + LPS stimulated RAW cell lysate
Performed under reducing conditions.
Predicted band size: 12 kDaMIF detected by Western Blot in LPS stimulated RAW cell lysate.
-
Western blot - Anti-MIF antibody (ab7207)From a review by Arsi Rosengren.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (38)
ab7207 は 38 報の論文で使用されています。
- Kantrong N et al. Macrophage migration inhibitory factor regulates specific innate immune sensor responses in gingival epithelial cells. J Periodontol 93:1940-1950 (2022). PubMed: 35100435
- Santos LC et al. Kisspeptin/Kiss1r system and angiogenic and immunological mediators at the maternal-fetal interface of domestic cats. Biol Reprod 105:217-231 (2021). PubMed: 33774655
- Gosuen Gonçalves Dias F et al. Topical formulations containing Copaifera duckei Dwyer oleoresin improve cutaneous wound healing. Avicenna J Phytomed 11:120-133 (2021). PubMed: 33907671
- Jin C et al. A Unique Type of Highly-Activated Microglia Evoking Brain Inflammation via Mif/Cd74 Signaling Axis in Aged Mice. Aging Dis 12:2125-2139 (2021). PubMed: 34881090
- Chen W et al. Exosomal MIF Derived From Nasopharyngeal Carcinoma Promotes Metastasis by Repressing Ferroptosis of Macrophages. Front Cell Dev Biol 9:791187 (2021). PubMed: 35036405