Anti-MIF 抗体 (ab34644)
Key features and details
- Chicken polyclonal to MIF
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgY
製品の概要
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製品名
Anti-MIF antibody
MIF 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to MIF -
由来種
Chicken -
特異性
The antibody is directed against the 12,300 MW human MIF protein and is useful in determining its presence in various assays. If neutralization experiments are performed for human MIF activity in bioassays, it is recommended to incubate the sample with a 1:500 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal chicken IgG is recommended. If FACS analysis experiments are performed for human MIF caution should be exhibited as the Fc domain of the chicken IgG molecule may interact with cells non-specifically.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment
交差が予測される動物種: Mouse, Rat, Sheep, Cow, Dog, Pig, Cynomolgus monkey, Macaque monkey
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免疫原
Synthetic peptide: PMFIVNTNV PRASVPDGFL SELTQQLAQA TG conjugated to KLH, corresponding to amino acids 2-32 of Human MIF
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ポジティブ･コントロール
- Supernatants or lysates of endotoxin-stimulated human peripheral blood mononuclear cells (PBMC).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
特記事項（精製）
This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34644の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Detects a band of approximately 12.3 kDa (predicted molecular weight: 14 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/2000. Detects a band of approximately 12.3 kDa (predicted molecular weight: 14 kDa). |
ターゲット情報
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機能
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. -
関連疾患
Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. -
配列類似性
Belongs to the MIF family. -
細胞内局在
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. - Information by UniProt
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参照データベース
- Entrez Gene: 280858 Cow
- Entrez Gene: 17319 Mouse
- Entrez Gene: 397412 Pig
- Entrez Gene: 81683 Rat
- Entrez Gene: 780466 Sheep
- SwissProt: P80177 Cow
- SwissProt: Q4R549 Cynomolgus monkey
- SwissProt: P34884 Mouse
see all -
別名
- GIF antibody
- GLIF antibody
- Glycosylation inhibiting factor antibody
see all
画像
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Western blot - Anti-MIF antibody (ab34644)All lanes : Anti-MIF antibody (ab34644) at 1/500 dilution
Lane 1 : Control
Lane 2 : 100 ng of recombinant
MIF present in a bacterial lysate
Secondary
Lane 2 : Goat anti chicken at 1/5000 dilution
Predicted band size: 14 kDa
Observed band size: 12.3 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (0)
ab34644 は論文での使用が確認できていません。
