Anti-MID1 抗体 (ab70770)
Key features and details
- Rabbit polyclonal to MID1
- Suitable for: ELISA, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MID1 antibody -
製品の詳細
Rabbit polyclonal to MID1 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide derived from an internal sequence of human MID1
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab70770 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70770の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
May have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation. -
組織特異性
In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain. -
関連疾患
Defects in MID1 are the cause of Opitz syndrome type I (OS-I) [MIM:300000]. OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules. -
配列類似性
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain.
Contains 1 COS domain.
Contains 1 fibronectin type-III domain.
Contains 1 RING-type zinc finger. -
翻訳後修飾
Phosphorylated on serine and threonine residues. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis. - Information by UniProt
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参照データベース
- Entrez Gene: 4281 Human
- Entrez Gene: 17318 Mouse
- Entrez Gene: 54252 Rat
- Omim: 300552 Human
- SwissProt: O15344 Human
- SwissProt: O70583 Mouse
- SwissProt: P82458 Rat
- Unigene: 27695 Human
see all -
別名
- BBBG 1 antibody
- BBBG1 antibody
- E3 ubiquitin-protein ligase Midline-1 antibody
see all
画像
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Western blot - Anti-MID1 antibody (ab70770)All lanes : Anti-MID1 antibody (ab70770) at 1/500 dilution
Lane 1 : 293 extract
Lane 2 : 293 extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 75 kDa
Observed band size: 75 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MID1 antibody (ab70770)ab70770 at 1/500 dilution staining MID1 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MID1 antibody (ab70770)ab70770 at 1/50 dilution staining MID1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab70770 は 9 報の論文で使用されています。
- Liang Y et al. Morphometric analysis of the size-adjusted linear dimensions of the skull landmarks revealed craniofacial dysmorphology in Mid1-cKO mice. BMC Genomics 24:68 (2023). PubMed: 36759768
- Ogura Y et al. Mid1 is associated with androgen-dependent axonal vulnerability of motor neurons in spinal and bulbar muscular atrophy. Cell Death Dis 13:601 (2022). PubMed: 35821212
- Chen X et al. Ubiquitin E3 ligase MID1 inhibits the innate immune response by ubiquitinating IRF3. Immunology 163:278-292 (2021). PubMed: 33513265
- Chen Q et al. TRIM18-Regulated STAT3 Signaling Pathway via PTP1B Promotes Renal Epithelial-Mesenchymal Transition, Inflammation, and Fibrosis in Diabetic Kidney Disease. Front Physiol 12:709506 (2021). PubMed: 34434118
- Qiao Y et al. MID1 and MID2 regulate cell migration and epithelial-mesenchymal transition via modulating Wnt/ß-catenin signaling. Ann Transl Med 8:1021 (2020). PubMed: 32953821
- Schweiger S et al. Resveratrol induces dephosphorylation of Tau by interfering with the MID1-PP2A complex. Sci Rep 7:13753 (2017). PubMed: 29062069
- Foong RE et al. Identification of genes differentially regulated by vitamin D deficiency that alter lung pathophysiology and inflammation in allergic airways disease. Am J Physiol Lung Cell Mol Physiol 311:L653-63 (2016). PubMed: 27496895
- Dierssen M et al. Reduced Mid1 Expression and Delayed Neuromotor Development in daDREAM Transgenic Mice. Front Mol Neurosci 5:58 (2012). WB ; Mouse . PubMed: 22563308
- Li T et al. Microcystin-LR (MCLR) Induces a Compensation of PP2A Activity Mediated by a4 Protein in HEK293 Cells. Int J Biol Sci 7:740-52 (2011). WB ; Human . PubMed: 21698000