Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-MICB 抗体 [EPR26362-65] (ab300485)

Submit a review Submit a question
Western blot - Anti-MICB antibody [EPR26362-65] (AB300485)
  • Western blot - Anti-MICB antibody [EPR26362-65] (ab300485)
  • Western blot - Anti-MICB antibody [EPR26362-65] (ab300485)
  • Flow Cytometry - Anti-MICB antibody [EPR26362-65] (AB300485)
  • Immunocytochemistry/ Immunofluorescence - Anti-MICB antibody [EPR26362-65] (ab300485)
  • Immunoprecipitation - Anti-MICB antibody [EPR26362-65] (AB300485)
  • Anti-MICB antibody [EPR26362-65] (AB300485)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26362-65] to MICB
  • Suitable for: IP, WB, Flow Cyt, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MICB antibody [EPR26362-65]
    MICB 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26362-65] to MICB

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WB, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Purified his-tagged human MICB recombinant protein. K-562, U266B1 whole cell lysate. K562 whole cell lysate treated with Protein Deglycosylation Mix II. ICC/IF: K-562 cells. Flow Cyt: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate. IP: K562 whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR26362-65

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300485の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/30.
WB
1/1000.
Flow Cyt
1/500.
ICC/IF
1/50.
特記事項
IP
1/30.
WB
1/1000.
Flow Cyt
1/500.
ICC/IF
1/50.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.

  • 組織特異性

    Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue.

  • 関連疾患

    Genetic variations in MICA are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=The MICB*004 allele is associated with rheumatoid arthritis.
    Note=Genetic variation in MICB is associated with cytomegalovirus and herpes simplex virus I seropositivity and this may be associated with schizophrenia risk.

  • 配列類似性

    Belongs to the MHC class I family. MIC subfamily.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • 翻訳後修飾

    Proteolytically cleaved and released from the cell surface of tumor cells.

  • 細胞内局在

    Cell membrane. Binding to human cytomegalovirus glycoprotein UL16 causes sequestration in the endoplasmic reticulum.
  • Information by UniProt

  • 参照データベース

    • 別名

      • MHC class I chain related protein B antibody
      • MHC class I chain related protein B2 antibody
      • MHC class I like molecule PERB11.2 IMX antibody
      • MHC class I mic B antigen antibody
      • MHC class I polypeptide related sequence B antibody
      • MHC class I polypeptide-related sequence B antibody
      • MIC B antibody
      • MIC-B antibody
      • MICB antibody
      • MICB_HUMAN antibody
      • PERB11.2 antibody
      • Stress inducible class I homolog antibody
      see all

    画像

    • Western blot - Anti-MICB antibody [EPR26362-65] (AB300485)
      Western blot - Anti-MICB antibody [EPR26362-65] (AB300485)
      All lanes : Anti-MICB antibody [EPR26362-65] (ab300485) at 1/1000 dilution

      Lane 1 : Purified his-tagged human MICB recombinant protein 10 ng
      Lane 2 : Purified his-tagged human MICA recombinant protein 10 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 37-50 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      This antibody does not cross-react with human MICA.

      Exposure time: 7.75 seconds. 

    • Western blot - Anti-MICB antibody [EPR26362-65] (ab300485)
      Western blot - Anti-MICB antibody [EPR26362-65] (ab300485)
      All lanes : Anti-MICB antibody [EPR26362-65] (ab300485) at 1/1000 dilution

      Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
      Lane 2 : U266B1 (human mtiple myeloma B lymphocyte) whole cell lysate
      Lane 3 : Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate
      Lane 4 : 293T (human embryonic kidney epithelial cell) whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Observed band size: 37-61 kDa why is the actual band size different from the predicted?


      Exposure time: 48 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:10359807).

      Negative control: Daudi, 293T (PMID: 28154561).

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Western blot - Anti-MICB antibody [EPR26362-65] (ab300485)
      Western blot - Anti-MICB antibody [EPR26362-65] (ab300485)
      All lanes : Anti-MICB antibody [EPR26362-65] (ab300485) at 1/1000 dilution

      Lane 1 : Untreated K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
      Lane 2 : K562 whole cell lysate treated with Protein Deglycosylation Mix II

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Observed band size: 37-61 kDa why is the actual band size different from the predicted?


      Exposure time: 103 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      MICB is a glycoprotein of approximately 61 kDa and detected as two 37-42 kDa bands after treated with Protein Deglycosylation MIX II. 

      The bands beneath the target band (37 kDa) are reported as deglycosylated cleaved soluble MICB. 

      Molecular weight observed is consistent with described in the literature (PMID:19147769).

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Flow Cytometry - Anti-MICB antibody [EPR26362-65] (AB300485)
      Flow Cytometry - Anti-MICB antibody [EPR26362-65] (AB300485)

      Flow cytometric analysis of K562 (human chronic myelogenous leukemia lymphoblast, Right) cells labelling MICB with ab300485 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: 293T (left), Daudi (middle) (PMID: 28154561).

    • Immunocytochemistry/ Immunofluorescence - Anti-MICB antibody [EPR26362-65] (ab300485)
      Immunocytochemistry/ Immunofluorescence - Anti-MICB antibody [EPR26362-65] (ab300485)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells lebelling MICB with ab300485 at 1/50 dilution (9.62 µg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in K-562 cell line. Negative control: No staining is observed in Daudi (PMID: 19404979 ).

      ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: PBS was used instead of primary antibody follwed by preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Immunoprecipitation - Anti-MICB antibody [EPR26362-65] (AB300485)
      Immunoprecipitation - Anti-MICB antibody [EPR26362-65] (AB300485)

      MICB was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 10 μg with ab300485 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300485 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 10 μg (Input)

      Lane 2: ab300485 IP in K562 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300485 in K562 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 180 seconds. 

      Observed MW (kDa): 61. 

    • Anti-MICB antibody [EPR26362-65] (AB300485)
      Anti-MICB antibody [EPR26362-65] (AB300485)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300485 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300485 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab300485.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com