AB279660

Anti-MICA + MICB 抗体 [6D4] - BSA and Azide free

Anti-MICA + MICB antibody [6D4] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal MICA antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

別名を表示する

PERB11.1, MICA, MHC class I polypeptide-related sequence A, MIC-A

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Flow Cytometry - Anti-MICA + MICB antibody [6D4] - BSA and Azide free (AB279660)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-MICA + MICB antibody [6D4] - BSA and Azide free (AB279660)

This data was developed using ab54413 the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / HeLa (Human cervix adenocarcinoma epithelial cell, Right) cells labelling MICA + MICB with ab54413 at 1/1000 dilution (0.1 μg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

Negative control : Jurkat.

Gated on viable cells.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

6D4

アイソタイプ

IgG2a

キャリアフリー

Yes

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

ab279660 is the carrier-free version of ab54413.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MICA and MICB also known as MIC-A and MIC-B are stress-induced proteins with significant roles in immune responses. These proteins are widely known for their presence on the surface of many human cell types most notably epithelial and endothelial cells. MICA is a glycoprotein with a molecular mass of approximately 70 kDa while MICB has a similar structure and mass. They are both part of the MHC class I-like molecules and function as ligands to the NKG2D receptor on natural killer (NK) cells and certain T cells a critical interaction for immune surveillance.

Biological function summary

MICA and MICB act in the activation of the immune system by modulating NK cells and CD8+ T cell activities. These proteins do not form part of a complex but operate independently to signal the presence of infected or transformed cells to the immune system. They often appear on the cell surface under stress such as during viral infections or cancerous transformations activating immune cells to target and destroy these abnormal cells.

Pathways

The MICA/MICB interaction with NKG2D is integral to the immune checkpoint pathway. This pathway plays a significant role in regulating the immune system’s response to aberrant cells. Additionally MICA and MICB activity is linked to the regulation of the cytokine IFN-γ promoting the recruitment and activation of immune effector cells. The roles of these proteins in these pathways underline their importance in maintaining immune homeostasis and responding to pathological challenges.

MICA and MICB have notable associations with cancer and autoimmune diseases. Aberrant expression of these proteins on tumor cells facilitates their recognition and elimination by immune cells linking MICA and MICB directly to tumor immunosurveillance. In autoimmune disorders improper regulation of MICA/MICB expression can lead to undesirable immune cell activation contributing to conditions like rheumatoid arthritis. The proteins connect with others like NKG2D in both cancer immunity and autoimmune diseases highlighting their dual role in health and disease.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed : 11491531, PubMed : 11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed : 10426993).

See full target information MICA

追加のターゲット

MICB

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MICA + MICB 抗体 [6D4] - BSA and Azide free

Anti-MICA + MICB antibody [6D4] - BSA and Azide free

Flow Cytometry - Anti-MICA + MICB antibody [6D4] - BSA and Azide free (AB279660)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise