Anti-MIC19 抗体 (ab154500)
Key features and details
- Rabbit polyclonal to MIC19
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MIC19 antibody
MIC19 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MIC19 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Pig
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免疫原
Recombinant fragment corresponding to Human MIC19 aa 1-214.
Database link: Q9NX63 -
ポジティブ･コントロール
- 293T, A431, Jurkat, Raji and mouse liver lysates; Hela xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154500の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Required for maintenance of mitochondrial crista integrity and mitochondrial function. May act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. -
組織特異性
Detected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue. -
配列類似性
Contains 1 CHCH domain. -
細胞内局在
Mitochondrion inner membrane. Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 616477 Cow
- Entrez Gene: 54927 Human
- Entrez Gene: 66075 Mouse
- Omim: 613748 Human
- SwissProt: Q5E9D3 Cow
- SwissProt: Q9NX63 Human
- SwissProt: Q9CRB9 Mouse
- Unigene: 655010 Human
see all -
別名
- CHCH3_HUMAN antibody
- CHCHD 3 antibody
- Chchd3 antibody
see all
画像
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Western blot - Anti-MIC19 antibody (ab154500)All lanes : Anti-MIC19 antibody (ab154500) at 1/2000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : MIC19 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 26 kDa -
Western blot - Anti-MIC19 antibody (ab154500)All lanes : Anti-MIC19 antibody (ab154500) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
12% SDS PAGE -
Western blot - Anti-MIC19 antibody (ab154500)Anti-MIC19 antibody (ab154500) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg
Predicted band size: 26 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIC19 antibody (ab154500)Immunohistochemical analysis of paraffin-embedded Hela xenograft, labeling MIC19 with ab154500 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154500 は 1 報の論文で使用されています。
- Habich M et al. Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay. Cell Rep 26:759-774.e5 (2019). PubMed: 30650365