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リコンビナントRabMAb

Anti-Mib1/Mindbomb 抗体 [EPR2762(2)] - BSA and Azide free (ab245766)

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OI-RD Scanning - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
  • Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2762(2)] to Mib1/Mindbomb - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free
    Mib1/Mindbomb 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2762(2)] to Mib1/Mindbomb - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WBmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Human
    非交差種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • K562, HeLa, Caco2, NCCIT, and Human brain lysates; Human kidney tissue.

  • 特記事項

    ab245766 is the carrier-free version of ab124929.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245766の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

 
WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
特記事項
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

 
WB
Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.

  • 組織特異性

    Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 配列類似性

    Contains 9 ANK repeats.
    Contains 2 MIB/HERC2 domains.
    Contains 3 RING-type zinc fingers.
    Contains 1 ZZ-type zinc finger.

  • 翻訳後修飾

    Ubiquitinated. Possibly via autoubiquitination.

  • 細胞内局在

    Cytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear.
  • Information by UniProt

  • 参照データベース

    • 別名

      • DAPK-interacting protein 1 antibody
      • Dip 1 antibody
      • DIP-1 antibody
      • Dip1 antibody
      • E3 ubiquitin protein ligase MIB 1 antibody
      • E3 ubiquitin protein ligase MIB1 antibody
      • E3 ubiquitin-protein ligase mib1 antibody
      • KIAA1323 antibody
      • LVNC7 antibody
      • MIB antibody
      • mib1 antibody
      • MIB1_HUMAN antibody
      • Mind bomb homolog 1 antibody
      • Mindbomb E3 ubiquitin protein ligase 1 antibody
      • Ubiquitin ligase mind bomb antibody
      • Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody
      • ZZANK2 antibody
      • ZZZ6 antibody
      see all

    画像

    • OI-RD Scanning - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
      OI-RD Scanning - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)

      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This image was produced using unpurified antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab124929).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)

      Immunohistochemical analysis of Human breast cancer tissue, staining Mib1/Mindbomb with ab124929.

      Tissue was fixed with paraformaldehyde and permeabilized with Tween buffer for 30 minutes at 20°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 9). Samples were incubated with primary antibody (1/5000 in diluent) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab124929).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)

      ab124929, at 1/250 dilution, staining Mib1/Mindbomb in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.

      This image was produced using unpurified antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab124929).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)

      ab124929 staining Mib1/Mindbomb in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

      This image was produced using purified antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab124929).

    • Immunocytochemistry/ Immunofluorescence - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
      Immunocytochemistry/ Immunofluorescence - Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)

      ab124929 staining Mib1/Mindbomb in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab124929 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

      Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
      Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

      This image was produced using purified antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab124929).

    • Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)
      Anti-Mib1/Mindbomb antibody [EPR2762(2)] - BSA and Azide free (ab245766)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245766 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245766 は論文での使用が確認できていません。

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