Anti-MHC Class II 抗体 [6C6] (ab55152)

The red is Wheat germ agglutinin (WGA). A widely used lectin in cell biology which has been conjugated to Alexa Fluor 594. WGA binds to sialic acid and N-acetylglucosaminyl residues.

Thank you, the images all arrived. I think you are correct, the TIFF files were probably too big.

Have you been able to obtain good stains for other proteins using this protocol?

I think the protocol is correct as it is. A customer did find that an enzymatic antigen retrieval gave a good MHC II stain of human tissue using ab55152 but I am not sure that will help with your samples. We do not know which enzyme he used but if you want to try an enzymatic antigen retrieval, I suggest using a trypsin solution. I can send more information about this if you like.The review can be found here:

https://www.abcam.com/MHC-Class-II-antibody-ab55152.html&tab=abreviews&intabreviewid=25380

Other antibodies may give better stains, assuming the samples have detectable amounts of CXCL14 and MHC class II. It is unlikely, though, that the EXPOSE kit ab80437 will improve the results.

I will be happy to send free replacements. I recommend the mouse monoclonal ab128065 for detection of MHC class II:

Click here (or use the following: https://www.abcam.com/MHC-Class-II-antibody-TDR311-ab128065.html).

The image on the datasheet is of a stain that uses a different substrate than what you are using but the antibody will work with your reagents, assuming you have an anti-mouse IgG secondary antibody. If not, please reply and I will make a recommendation for a compatible secondary antibody.

For CXCL14, I recommend the rabbit polyclonal ab36622:

Click here (or use the following: https://www.abcam.com/CXCL14-antibody-ab36622.html).

If you are using biotinylated secondary antibodies, you may want to consider using an endogenous biotin blocking kit. Your negative controls slides appear to have high background, possibly from endogenous biotin, and this may obscure the signal from CXCL14 and MHC II. We have a product for this, ab3387:

Click here (or use the following: https://www.abcam.com/Endogenous-Avidin--Biotin-Blocking-System-ab3387.html).

Please let me know if you need more information, and if you want to try the two antibodies that I recommend.

Thank you for contacting us.


I have searched our catalog and unfortunately we do not currently have any anti-MHC Class II antibodies which have been tested in hamster. Looking further at the hamster protein against the mouse, rat and human proteins I found that the best homologies tend to be with human and would therefore recommend using a product raised against the human protein or part of it. We do have a few products which would be appropriate, namely ab116378 and ab55152.

If you are interested in testing these in hamster I could enroll you in our Abreview 100% discount program if you buy an anti MHC class II antibody now, test it in hamster and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and which product you would like to test. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Test it in hamster.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for hamster, you will still receive the discount on your next purchase after your Abreview has been submitted.


Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab116378. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.