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AB33752

Anti-MHC class I RT Ia 抗体 [OX-18]

Anti-MHC class I RT Ia antibody [OX-18]

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(4 Publications)

Mouse Monoclonal MHC class I RT Ia antibody. Suitable for IP, Flow Cyt, ELISA, IHC-Fr and reacts with Rat samples. Cited in 4 publications.

別名を表示する

RT1 class I gene, RT1 class I gene (A 1), RT1 class Ia locus A, RT1 class Ia, locus A (A1), RT1 class Ia, locus A1, RT1.A1(N), RT1A 1

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

OX-18

アイソタイプ

IgG1

キャリアフリー

No

交差種

Rat

アプリケーション

IP, Flow Cyt, ELISA, IHC-Fr

applications

特異性

Ab33752 recognises a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 10ul of this dilution for 10^6 cells in 100ul. <a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>Acetone fixation recommended. The antigen is sensitive to fixation with paraformaldehyde.</p>" } } }

製品の詳細

This clone (OX-18) has been used in immunoaffinity purification of rat MHC class I molecules.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
精製に関する特記事項
This antibody was purified tissue culture supernatant.
バッファー組成
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MHC class I RT Ia also known as RT1-A1 mechanically presents peptide antigens to CD8+ T cells. This protein has a mass of approximately 45 kDa. Expression of this protein occurs widely across various cell types including most nucleated cells. It plays an important role in the immune surveillance mechanism by displaying antigenic peptides on the cell surface for recognition by T-cell receptors.
Biological function summary

MHC class I RT Ia interacts with peptides within the endoplasmic reticulum and forms a complex with beta-2 microglobulin. This interaction is essential for the stability of the peptide-MHC complex on the cell surface. The formation of these complexes allows for immune recognition and establishment of immune responses. MHC class I RT Ia therefore plays an important role in initiating immune reactions against intracellular pathogens such as viruses.

Pathways

MHC class I RT Ia participates in the antigen processing and presentation pathway a fundamental aspect of adaptive immunity. It interacts with the transporter associated with antigen processing (TAP) proteins which transport peptides to the endoplasmic reticulum. Apart from that it also engages in the pathway involving cytotoxic T lymphocyte activation where it directly influences T-cell receptor signaling.

MHC class I RT Ia connects to autoimmune diseases like type 1 diabetes and ankylosing spondylitis. Variations in the genes encoding for MHC class I molecules may predispose individuals to these conditions. Additionally it’s associated with the HIV-1 virus where the virus may escape immune detection by downregulating MHC class I molecules presenting challenges in immune response which affect viral loads and disease progression.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Experimental neurology 282:78-85 PubMed27235932

2016

hESC-derived neural progenitors prevent xenograft rejection through neonatal desensitisation.

Applications

IHC

Species

Rat

Andreas Heuer,Agnete Kirkeby,Ulrich Pfisterer,Marie E Jönsson,Malin Parmar

Immunology letters 135:118-23 PubMed20974178

2010

Major histocompatibility complex class I-mediated inhibition of neurite outgrowth from peripheral nerves.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongqi-Phyllis Wu,Tina Bilousova,Nathalie Escande-Beillard,Hoa Dang,Terry Hsieh,Jide Tian,Daniel L Kaufman

Transplantation proceedings 25:2806-7 PubMed8212242

1993

Preincubation of tumor cells with the anti-(MHC) class I antibody OX18 enhances tumor cell lysis by interleukin-2-activated NK cells.

Applications

Unspecified application

Species

Unspecified reactive species

K M Smits,P J Kuppen,A M Eggermont,G J Fleuren

European journal of immunology 12:237-43 PubMed6178598

1982

Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus.

Applications

Unspecified application

Species

Unspecified reactive species

T Fukumoto,W R McMaster,A F Williams
View all publications

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