AB102126

Anti-mH2A2 抗体

Name: Anti-mH2A2 抗体 (ab102126) | Abcam
Brand: Abcam Limited
SKU: AB102126
Price: 76000 JPY
Availability: InStock
Rating: 2 (4 reviews)

Anti-mH2A2 antibody

(4 Reviews)

(4 Publications)

Rabbit Polyclonal mH2A2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MACROH2A2 aa 150-350.

別名を表示する

H2AFY2, MACROH2A2, Core histone macro-H2A.2, Histone macroH2A2, mH2A2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB102126)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB102126)

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using ab102126 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB102126)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue labelling Macro H2A.2 protein at nucleus with ab102126 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human, Mouse

アプリケーション

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human MACROH2A2 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q9P0M6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MH2A2 also known as macroH2A2 is a variant of the histone H2A family with a distinct structural feature that includes a large macro domain. This protein weighs approximately 42 kDa and plays roles in chromatin organization and transcription regulation. The expression of mH2A2 occurs in various tissues with notable presence in the liver and testis. It acts by incorporating itself into nucleosomes affecting chromatin accessibility and influencing gene expression.

Biological function summary

MH2A2 influences processes such as gene silencing DNA repair and cellular differentiation. It is part of the nucleosome complex where its presence can result in a more compact chromatin structure leading to decreased gene activity. mH2A2 contributes to X-chromosome inactivation in females by associating with the inactive X chromosome. Moreover it has a role in the cellular response to DNA damage where it helps in maintaining genome stability.

Pathways

MH2A2 is involved in the chromatin remodeling and transcription regulation pathways. It interacts with proteins like histone deacetylases (HDACs) and polycomb repressive complexes impacting epigenetic gene regulation. Through these pathways mH2A2 participates in controlling developmental processes and cellular differentiation linking its function to the A/B compartments of chromatin. This regulation ultimately influences cell-specific gene expression patterns and cellular identity.

MH2A2 has been linked to cancer and metabolic disorders. Studies have shown altered expression of mH2A2 in certain cancers where it associates with tumor suppressor proteins like p53 impacting cellular growth and apoptosis. In metabolic disorders changes in mH2A2 levels affect liver metabolism potentially contributing to conditions such as non-alcoholic fatty liver disease (NAFLD). Understanding the role of mH2A2 in these diseases could provide insights into developing novel therapeutic strategies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.

See full target information MACROH2A2

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

PloS one 18:e0288005 PubMed37432970

2023

Combinatorial targeting of a specific EMT/MET network by macroH2A variants safeguards mesenchymal identity.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitrios Valakos,Eleftheria Klagkou,Antonis Kokkalis,Alexandros Polyzos,Fotis L Kyrilis,Aggelos Banos,Giannis Vatsellas,Maria Pliatska,Ethan Ford,Dimitrios J Stravopodis,Dimitris Thanos

Nucleic acids research 49:8024-8036 PubMed34223906

2021

LSH catalyzes ATP-driven exchange of histone variants macroH2A1 and macroH2A2.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ni,Kathrin Muegge

Nature communications 12:3520 PubMed34112784

2021

The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Xu,Kai Ni,Yafeng He,Jianke Ren,Chongkui Sun,Yie Liu,Mirit I Aladjem,Sandra Burkett,Richard Finney,Xia Ding,Shyam K Sharan,Kathrin Muegge

Nucleic acids research 44:5148-60 PubMed26939888

2016

Chromosome intermingling-the physical basis of chromosome organization in differentiated cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shovamayee Maharana,K Venkatesan Iyer,Nikhil Jain,Mallika Nagarajan,Yejun Wang,G V Shivashankar

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-mH2A2 抗体

Anti-mH2A2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB102126)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB102126)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise