Anti-MGMT 抗体 [MT3.1] (ab39253)

Thank you for contacting Abcam.

We do not have expiration dates for our products, but we do guarantee our products for 6 months if stored according to the datasheet. Although we cannot guarantee it, based on our in-house testing if the antibody is stored at 4C, then it should be good for a year.

If there is anything else I can help you with, please let me know.

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Thank you very much for your reply. My colleague is out of the office today but I'll be happy to help you with this.

We don't currently have blocking peptides available for ab105340 or ab56540, but I'm in the process of adding the peptide for ab105340 to our catalog for you. Since ab56540 was raised against the full length protein instead of a short peptide, there is no blocking peptide available for this antibody.

Blocking peptides are often used in Western blot to verify specificity of a band or bands. The amino acid sequence of the blocking peptide is the same as that of the immunogenic peptide. By pre-treating the antibody with an excess of the blocking peptide, the specific binding sites of the antibody should be "blocked", so that if the antibody-peptide complex is used in the Western blot instead of the pure antibody, the specific bands will not appear on the blot. Here is the blocking protocol, just for further clarification of the concept-

https://www.abcam.com/index.html?pageconfig=resource&rid=11378

You couldin theoryuse a full-length protein to block ab56540, but this requires quite a lot of protein and may not be successful or give conclusive results. Knock-out testing or mass spectrometry of the suspected band may be a more efficient and definitive control.

I hope that this will be helpful. We will be back in touch as soon as we have the blocking peptide of ab105340 available for you.

Thank you for your reply. The publications that are known to reference an Abcam product can be found on our online datasheets on the specific references tab. Below are PubMed IDs for the antibodies you are interested in.

MGMT (ab39253) - 21752281, 17508213

ABH2 (ab105340) - no known references

APNG (ab55461) - 22496614, 20064251

ABH3 (ab56540) - no known references

I hope this helps, please let me know if you need any additional information or assistance.

Thank you for contacting us.

The final dilutions should be optimized for your particular samples and protocols, but the following would be my recommended starting dilutions for WB with an overnight incubation:

MGMT (ab39253) - 1:500

ABH2 (ab105340) - 1:1000

APNG (ab55461) - 1:500

ABH3 (ab56540) - 1:500

I hope this helps, please let me know if you need any additional information or assistance.

Thank you for contacting us and sorry for the delay in getting back to you.


I can confirm that theaverage concentration of this antibody is 66.6 ug/ml.


I hope this information is of help. If you require any further information please do not hesitate to contact us again.