Anti-mGluR1 抗体 (ab82211)
Key features and details
- Rabbit polyclonal to mGluR1
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-mGluR1 antibody
mGluR1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to mGluR1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide corresponding to Human mGluR1 aa 1159-1171.
Sequence:SSVPSSPVSESVL
Database link: Q13255 -
ポジティブ･コントロール
- WB: Human brain lysate. ICC/IF: Rat brain (cingulate cortex).
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab82211の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa).
|
|
| ICC/IF |
1/100 - 1/500.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa). |
|
ICC/IF
1/100 - 1/500. |
ターゲット情報
-
機能
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. -
配列類似性
Belongs to the G-protein coupled receptor 3 family. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 2911 Human
- Entrez Gene: 24414 Rat
- Omim: 604473 Human
- SwissProt: Q13255 Human
- SwissProt: P23385 Rat
- Unigene: 32945 Human
- Unigene: 87787 Rat
-
別名
- Glutamate receptor metabotropic 1 antibody
- glutamate receptor, metabotropic 1 antibody
- GPRC1A antibody
see all
画像
-
Western blot - Anti-mGluR1 antibody (ab82211)Anti-mGluR1 antibody (ab82211) at 1/1000 dilution + human brain lysate at 20 µg
Secondary
AP-conjugated secondary at 1/5000 dilution
Predicted band size: 133 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-mGluR1 antibody (ab82211)Immunofluorescence labeling of mGluR1 in rat brain (cingulate cortex). Using ab82211 (red color). DAPI was used to counter-stain cell nuclei (blue color). Working dilution is 1/100-1/500.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (18)
ab82211 は 18 報の論文で使用されています。
- Luo XD et al. Activation of metabotropic glutamate receptor 1 regulates hippocampal CA1 region excitability in rats with status epilepticus by suppressing the HCN1 channel. Neural Regen Res 18:594-602 (2023). PubMed: 36018183
- Hagena H et al. Lifelong changes of neurotransmitter receptor expression and debilitation of hippocampal synaptic plasticity following early postnatal blindness. Sci Rep 12:9142 (2022). PubMed: 35650390
- Alhaddad H et al. Effects of a Novel Beta Lactam Compound, MC-100093, on the Expression of Glutamate Transporters/Receptors and Ethanol Drinking Behavior of Alcohol-Preferring Rats. J Pharmacol Exp Ther 383:208-216 (2022). PubMed: 36153003
- Yue S et al. Insulin-like growth factor 1 regulates excitatory synaptic transmission in pyramidal neurons from adult prefrontal cortex. Neuropharmacology 217:109204 (2022). PubMed: 35931212
- Batool S et al. Spatiotemporal Patterns of Menin Localization in Developing Murine Brain: Co-Expression with the Elements of Cholinergic Synaptic Machinery. Cells 10:N/A (2021). PubMed: 34065662